Development of Saccharomyces cerevisiae as a model pathogen. A system for the genetic identification of gene products required for survival in the mammalian host environment.

Genetics
A L Goldstein, J H McCusker

Abstract

Saccharomyces cerevisiae, a close relative of the pathogenic Candida species, is an emerging opportunistic pathogen. An isogenic series of S. cerevisiae strains, derived from a human clinical isolate, were used to examine the role of evolutionarily conserved pathways in fungal survival in a mouse host. As is the case for the corresponding Candida albicans and Cryptococcus neoformans mutants, S. cerevisiae purine and pyrimidine auxotrophs were severely deficient in survival, consistent with there being evolutionary conservation of survival traits. Resistance to the antifungal drug 5-fluorocytosine was not deleterious and appeared to be slightly advantageous in vivo. Of mutants in three amino acid biosynthetic pathways, only leu2 mutants were severely deficient in vivo. Unlike the glyoxylate cycle, respiration was very important for survival; however, the mitochondrial genome made a respiration-independent contribution to survival. Mutants deficient in pseudohyphal formation were tested in vivo; flo11Delta mutants were phenotypically neutral while flo8Delta, tec1Delta, and flo8Delta tec1Delta mutants were slightly deficient. Because of its ease of genetic manipulation and the immense S. cerevisiae database, which includes the bes...Continue Reading

References

Jan 1, 1979·Sub-cellular Biochemistry·P A Whittaker
Aug 2, 1976·Molecular & General Genetics : MGG·S G Oliver, D H Williamson
Aug 2, 1976·Molecular & General Genetics : MGG·S G Oliver, D H Williamson
Mar 1, 1992·Infection and Immunity·A VarmaK J Kwon-Chung
Mar 15, 1992·European Journal of Biochemistry·E FernándezR Rodicio
Sep 1, 1991·Infection and Immunity·D R Kirsch, R R Whitney
Sep 1, 1990·Microbiological Reviews·A Tzagoloff, C L Dieckmann
Apr 23, 1971·Nature·R B Flavell
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·V SmithP O Brown
Feb 1, 1995·Infection and Immunity·J K ByronD A Stevens
Oct 1, 1994·Trends in Microbiology·H Vanden BosscheF C Odds
Apr 1, 1994·The Journal of Infectious Diseases·K V ClemonsD A Stevens
Oct 1, 1995·Clinical Microbiology Reviews·K C Hazen
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M G LambrechtsI S Pretorius
Jan 1, 1996·Annual Review of Biochemistry·R O Poyton, J E McEwen
Jul 25, 1997·The Journal of Biological Chemistry·D M GlerumA Tzagoloff
Sep 23, 1997·Cell·H J LoG R Fink
Feb 18, 1998·Journal of Clinical Microbiology·M J McCulloughD A Stevens
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BjörkmanD I Andersson
Jun 20, 1998·Clinical Microbiology Reviews·T C WhiteR A Bowden
Aug 26, 1998·Science·E A WinzelerR W Davis

❮ Previous
Next ❯

Citations

Jan 30, 2002·Yeast
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Carol W MooreRonald E Gordon
Sep 14, 2002·Infection and Immunity·Thomas H RudeJohn R Perfect
Jan 24, 2007·The Journal of Antimicrobial Chemotherapy·André A WilliamsRichard D Gandour
Nov 16, 2007·Organic & Biomolecular Chemistry·David MormeneoAntonio Delgado
Nov 21, 2007·Medical Mycology·Javier CapillaDavid A Stevens
Dec 1, 2004·Annual Review of Genetics·Elizabeth Pradel, Jonathan J Ewbank
Aug 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Gordon ChuaTimothy R Hughes
Nov 29, 2002·Eukaryotic Cell·Michael C Lorenz, Gerald R Fink
Jul 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wu WeiLars M Steinmetz
Apr 13, 2004·Eukaryotic Cell·Yiorgos ApidianakisEleftherios Mylonakis
Feb 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Robert T WheelerGerald R Fink
Jul 27, 2002·Annual Review of Microbiology·Leah E CowenLinda M Kohn
Oct 5, 2010·Bioorganic & Medicinal Chemistry Letters·Ki-Bong OhHyi-Seung Lee
Sep 21, 2007·Archives of Pharmacal Research·Hyeong-Cheol YangSanghee Kim
Jan 27, 2009·Bioorganic & Medicinal Chemistry Letters·Hyi-Seung LeeKi-Bong Oh
Jul 27, 2010·PloS One·Erica M Larson, Alexander Idnurm
Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey M BeckerTerry Roemer
May 31, 2012·Genome Research·Hunter B FraserHimanshu Sinha
Feb 25, 2011·Astrobiology·Sebastiaan E Van MuldersRonnie Willaert

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.