Development of sandwich ELISA for detection and quantification of invertebrate major allergen tropomyosin by a monoclonal antibody

Food Chemistry
Hong ZhangKazuo Shiomi

Abstract

Muscle protein tropomyosins of invertebrates are major allergens responsible for wide spread allergic reactions against invertebrates such as shellfish and insects. In order to develop a sandwich enzyme-linked immunoadsorbent assay (ELISA) for detection and quantification of the invertebrate pan-allergen tropomyosin, a specific monoclonal antibody (MAb), CE7B2, was produced. We have successfully established a sandwich ELISA for measuring invertebrate tropomyosin concentrations in food and food materials. The sandwich ELISA system using the MAb CE7B2 is a useful tool to detect and quantify levels of tropomyosin in food. The method is also helpful to detect mite and cockroach tropomyosins, the important indoor allergens.

References

Nov 1, 1994·The Journal of Allergy and Clinical Immunology·P S LeungM E Gershwin
Feb 1, 1996·Current Opinion in Cell Biology·V M Fowler
Aug 1, 1997·The Journal of Allergy and Clinical Immunology·B J JeoungS B Lehrer
Sep 4, 1999·International Archives of Allergy and Immunology·G ReeseS B Lehrer
Mar 15, 2002·International Archives of Allergy and Immunology·R AyusoG Reese
Oct 10, 2002·International Archives of Allergy and Immunology·Rosalia AyusoSamuel B Lehrer
Apr 20, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B BarlettaG Di Felice
Aug 11, 2005·Current Allergy and Asthma Reports·L Karla Arruda
Aug 21, 2007·Journal of Molecular Biology·Alla S KostyukovaNorma J Greenfield
Oct 13, 2007·Journal of Agricultural and Food Chemistry·Kosuke SeikiYasuo Ohno
Apr 24, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A L LopataS B Lehrer

❮ Previous
Next ❯

Citations

Feb 15, 2016·Current Allergy and Asthma Reports·Julia ZimmerSusanne Kaul
Nov 11, 2014·Journal of Agricultural and Food Chemistry·Margaret R BakerQing X Li
Jun 1, 2018·Critical Reviews in Food Science and Nutrition·Mati Ullah KhanYong-Ning Wu
Dec 31, 2020·International Journal of Molecular Sciences·Shaymaviswanathan KarnaneediAndreas L Lopata
Jun 30, 2021·Critical Reviews in Food Science and Nutrition·Jilin LiJun-Hu Cheng
Apr 13, 2018·Journal of Agricultural and Food Chemistry·Bo FuQing X Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.