PMID: 9549062Apr 29, 1998Paper

Development of sequence characterized DNA markers linked to a dominant verticillium wilt resistance gene in tomato

Genome Génome / Conseil National De Recherches Canada
L M KawchukD R Lynch

Abstract

Sequences were determined for codominant RAPD markers closely linked to the Ve locus, a dominant verticillium wilt resistance gene in tomato. Analysis of the sequences linked to Ve and ve revealed a perfectly homologous sequence with a central polymorphic region comprising 79 nucleotide substitutions, insertions, and deletions. Codominant and allele-specific SCARs were developed using conserved and polymorphic sequences linked to the Ve locus. High resolution linkage analysis using F2 progeny segregating for resistance and marker-assisted selection indicated that linkage between the genetic markers and the Ve locus is less than 0.67 +/- 0.49 cM. Sequences were useful in determining the molecular structure of a polymorphic genomic region closely linked to the Ve locus and in developing genetic markers that facilitated marker-assisted selection of the resistant, susceptible, heterozygous, and homozygous genotypes.

Citations

May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·L M KawchukD Prufer
Jan 5, 2001·The Journal of Eukaryotic Microbiology·E Orias
Jun 5, 2002·The Journal of Eukaryotic Microbiology·Jeffrey S FillinghamRonald E Pearlman
Apr 24, 2009·Annual Review of Phytopathology·Steven J KlostermanKrishna V Subbarao
Mar 28, 2008·International Journal of Plant Genomics·Majid R Foolad
Aug 18, 2006·BMC Evolutionary Biology·Chunguang DuJoachim Messing
Oct 27, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Paul ArensBen Vosman
Jan 1, 2005·Molecular Plant Pathology·Marco KruijtPierre J G M de Wit

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