Development of SRAP, SRAP-RGA, RAPD and SCAR markers linked with a Fusarium wilt resistance gene in eggplant.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Nedim MutluKazim Abak

Abstract

Fusarium wilt (Fusarium oxysporum Schlecht. f. sp. melongenae) is a vascular disease of eggplant (Solanum melongena L.). The objectives of this work were (1) to confirm the monogenic inheritance of fusarium wilt resistance in eggplant, (2) to identify molecular markers linked to this resistance, and (3) to develop SCAR markers from most informative markers. We report the tagging of the gene for resistance to fusarium wilt (FOM) in eggplant using SRAP, RGA, SRAP-RGA and RAPD markers. Analysis of segregation data confirmed the monogenic inheritance of resistance. DNA from F(2) and BC(1) populations of eggplant segregating for fusarium wilt resistance was screened with 2,316 primer combinations to detect polymorphism. Three markers were linked within 2.6 cM of the gene. The codominant SRAP marker Me8/Em5 and dominant SRAP-RGA marker Em12/GLPL2 were tightly linked to each other and mapped 1.2 cM from the resistance gene, whereas RAPD marker H12 mapped 2.6 cM from the gene and on the same side as the other two markers. The SRAP marker was converted into two dominant SCAR markers that were confirmed to be linked to the resistance gene in the F(2,) BC(1) and F(2) of BC(3) generations of the same cross. These markers provide a starting...Continue Reading

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Citations

Jun 10, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Peizheng WangTianzhen Zhang
Feb 10, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yves CastonguaySerge Laberge
Apr 15, 2010·Biochemical Genetics·O BaysalJaime A Teixeira da Silva
Oct 22, 2010·Genome Génome / Conseil National De Recherches Canada·Lorenzo BarchiGiuseppe Leonardo Rotino
Oct 9, 2013·Genome Génome / Conseil National De Recherches Canada·Juansheng RenGuangjun Ren
Nov 12, 2013·Biochemical Genetics·Shang-Guo FengHui-Zhong Wang
Feb 5, 2016·Journal of Microbiological Methods·Mariana G GraçaAnna S Levin
Nov 20, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Koji MiyatakeHiroyuki Fukuoka
Apr 30, 2009·Molecular Plant Pathology·Caroline B Michielse, Martijn Rep
Jun 19, 2013·Molecular Phylogenetics and Evolution·Rachel S MeyerAmy Litt
Jun 10, 2010·Journal of Agricultural and Food Chemistry·Giuseppe MennellaRoberto Lo Scalzo

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