Development of Stable Homozygous Wheat/Amblyopyrum muticum (Aegilops mutica ) Introgression Lines and Their Cytogenetic and Molecular Characterization

Frontiers in Plant Science
Julie KingIan P King

Abstract

Wheat is one of the world's most important sources of food. However, due to its evolution its genetic base has narrowed, which is severely limiting the ability of breeders to develop new higher yielding varieties that can adapt to the changing environment. In contrast to wheat, its wild relatives provide a vast reservoir of genetic variability for most, if not all, agronomically important traits. Genetic variation has previously been transferred to wheat from one of its wild relatives, Ambylopyrum muticum (previously known as Aegilops mutica). However, before the genetic variation available in this species can be assessed and exploited in breeding and for research, the transmission of the chromosome segments introgressed into wheat must first be stabilized. In this paper we describe the generation of 66 stably inherited homozygous wheat/Am. muticum introgression lines using a doubled haploid procedure. The characterisation and stability of each of these lines was determined via genomic in situ hybridization and SNP analysis. While most of the doubled haploid lines were found to carry only single introgressions, six lines carried two. Three lines carried only complete Am. muticum chromosomes, 43 carried only small or very small ...Continue Reading

References

Sep 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Akio KatoJames A Birchler
Jun 23, 2009·Genome Research·Martin KrzywinskiMarco A Marra
Mar 8, 2011·Comptes rendus biologies·Gilles Charmet
Aug 7, 2012·Plant, Cell & Environment·Matthew ReynoldsGustavo Slafer
Sep 5, 2012·BMC Bioinformatics·Paul A WilkinsonKeith J Edwards
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Huakun ZhangBao Liu
Jun 28, 2016·BMC Bioinformatics·Paul A WilkinsonKeith J Edwards
Jul 28, 2016·Plant Biotechnology Journal·Julie KingIan P King

❮ Previous
Next ❯

Citations

Aug 1, 2020·Frontiers in Plant Science·Radim SvačinaJan Bartoš
Mar 27, 2020·Frontiers in Plant Science·Ming HaoDengcai Liu
May 21, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·James SteadhamNidhi Rawat

❮ Previous
Next ❯

Methods Mentioned

BETA
environmental stresses
Genotyping

Software Mentioned

Axiom
McGISH
Meta Systems ISIS
Metafer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.