Development of striatal patch/matrix organization in organotypic co-cultures of perinatal striatum, cortex and substantia nigra

Neuroscience
A Snyder-KellerDavid J Graber

Abstract

Organotypic cultures of fetal or early postnatal striatum were used to assess striatal patch formation and maintenance in the presence or absence of dopaminergic and glutamatergic influences. Vibratome-cut slices of the striatum prepared from embryonic day 19 to postnatal day 4 rat pups were maintained in static culture on clear membrane inserts in Dulbecco's modified Eagle's medium/F12 (1:1) with 20% horse serum. Some were co-cultured with embryonic day 12-16 ventral mesencephalon and/or embryonic day 19 to postnatal day 4 cortex, which produced a dense dopaminergic innervation and a modest cortical innervation. Donors of striatal and cortical tissue were previously injected with bromo-deoxyuridine (BrdU) on embryonic days 13 and 14 in order to label striatal neurons destined to populate the patch compartment of the striatum. Patches of BrdU-immunoreactive cells were maintained in organotypic cultures of late prenatal (embryonic days 20-22) or early postnatal striatum in the absence of nigral dopaminergic or cortical glutamatergic influences. In slices taken from embryonic day 19 fetuses prior to the time of in vivo patch formation, patches were observed to form after 10 days in vitro, in 39% of nigral-striatal co-cultures com...Continue Reading

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Citations

May 22, 2010·Histochemistry and Cell Biology·S C SchockGordon W Arbuthnott
Jun 26, 2012·Physiology & Behavior·Linda M BartoshukDerek J Snyder
Nov 23, 2006·Journal of Biomedical Materials Research. Part a·Matthew R HyndWilliam Shain
Apr 26, 2008·Synapse·Abigail Snyder-KellerPatricio O'Donnell
Aug 9, 2015·Neuroscience·C Humpel
Aug 13, 2011·Journal of Neuroscience Methods·Celine UllrichChristian Humpel
Jun 26, 2009·Progress in Neurobiology·Laura LossiAdalberto Merighi

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