Development of Structure-Switching Aptamers for Kanamycin Detection Based on Fluorescence Resonance Energy Transfer

Frontiers in Chemistry
Xinyue MaMingdi Zhang

Abstract

The structure-switching aptamers are designed for the simple and rapid detection of kanamycin based on the signal transduction principle of fluorescence resonance energy transfer (FRET). The structure switch is composed of kanamycin-binding aptamers and the complementary strands, respectively labeled with fluorophore and quencher, denoted as FDNA and QDNA. In the absence of kanamycin, FDNA and QDNA form the double helix structure through the complementary pairing of bases. The fluorophore and the quencher are brought into close proximity, which results in the fluorescence quenching because of the FRET mechanism. In the presence of kanamycin, the FDNA specifically bind to the target due to the high affinity of aptamers, and the QDNA are dissociated. The specific recognition between aptamers and kanamycin will obstruct the formation of structure switch and reduce the efficiency of FRET between FDNA and QDNA, thus leading to the fluorescence enhancement. Therefore, based on the structure-switching aptamers, a simple fluorescent assay for rapid detection of kanamycin was developed. Under optimal conditions, there was a good linear relationship between kanamycin concentration and the fluorescence signal recovery. The linear range of...Continue Reading

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Mar 1, 1996·Nature Biotechnology·S Tyagi, F R Kramer
Jan 23, 2003·Journal of Agricultural and Food Chemistry·Elma E M G LoomansAart Van Amerongen
Apr 17, 2003·Journal of the American Chemical Society·Razvan Nutiu, Yingfu Li
Jan 9, 2004·Infectious Disease Clinics of North America·John Turnidge
Apr 14, 2004·Chemistry : a European Journal·Razvan Nutiu, Yingfu Li
Dec 1, 2007·Analytical and Bioanalytical Chemistry·Manjula Rajendran, Andrew D Ellington
Jun 28, 2008·Biophysical Chemistry·Yan Liu, Rong Guo
May 3, 2011·Analytical Biochemistry·Kyung-Mi SongChangill Ban
Mar 6, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Mustapha OuanezarAntoine Bouchoux
May 26, 2018·Current Opinion in Structural Biology·Kenji Okamoto, Yasushi Sako
Aug 7, 2018·Analytical Biochemistry·Sathya SrinivasanBhaskar Mohan Murari
May 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bing ChenFeng Wang

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Methods Mentioned

BETA
electrophoresis
surface plasmon resonance
biosensors
fluorescence resonance
FRET
protein concentration assay
Assay

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