Development of STS and CAPS markers for variety identification and genetic diversity analysis of tea germplasm in Taiwan

Botanical Studies
Chih-Yi HuShun-Fu Lin

Abstract

Tea (Camellia sinensis) is an important economic crop in Taiwan. Particularly, two major commercial types of tea (Paochong tea and Oolong tea) which are produced in Taiwan are famous around the world, and they must be manufactured with specific cultivars. Nevertheless, many elite cultivars have been illegally introduced to foreign countries. Because of the lower cost, large amount of "Taiwan-type tea" are produced and imported to Taiwan, causing a dramatic damage in the tea industry. It is very urgent to develop the stable, fast and reliable DNA markers for fingerprinting tea cultivars in Taiwan and protecting intellectual property rights for breeders. Furthermore, genetic diversity and phylogenetic relationship evaluations of tea germplasm in Taiwan are imperative for parental selection in the cross-breeding program and avoidance of genetic vulnerability. Two STS and 37 CAPS markers derived from cytoplasmic genome and ESTs of tea have been developed in this study providing a useful tool for distinguishing all investigated germplasm. For identifying 12 prevailing tea cultivars in Taiwan, five core markers, including each one of mitochondria and chloroplast, and three nuclear markers, were developed. Based on principal coordinat...Continue Reading

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Citations

Mar 12, 2020·Database : the Journal of Biological Databases and Curation·Himanshu DubeyTapan Kumar Mondal
Jul 30, 2019·Journal of Food Biochemistry·Yue-Chiun LiJason T C Tzen

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SeqMan Pro
PowerMarker
NTSYSpc
Primer3
SNP2CAPS
TFPGA
Popgene

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