Development of the GABA system in organotypic culture of hippocampal and cerebellar slices from a 67-kDa isoform of glutamic acid decarboxylase (GAD67)-deficient mice

Neuroscience Research
F Ji, K Obata

Abstract

Mice lacking glutamic acid decarboxylase 67kDa (GAD67) are born with much reduced GABA contents but do not survive after birth. To estimate the subsequent development of GAD67-deficient neural tissue, the hippocampal and cerebellar slices were prepared while the mice were alive, and subjected to organotypic culture. Although GABA contents were initially less than 5%, they were markedly increased, and GABA-containing fiber networks were well developed during the culture, indicating that GAD65, the other GAD isoform, largely compensates for the lack of GAD67. Cerebellar Purkinje cells developed similarly in culture.

References

May 31, 2012·Acta neurologica Belgica·Qiao-Zhi Zou, Xiu-Li Shang
Jun 23, 2010·Neural Development·Timm SchubertRachel O L Wong
Apr 12, 2013·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Kunihiko Obata
Sep 10, 2011·Neural Plasticity·Guillermo Gonzalez-BurgosDavid A Lewis
Jun 10, 2008·The Journal of Comparative Neurology·Alcmène ChalazonitisMichael D Gershon
Apr 10, 2009·Autism Research : Official Journal of the International Society for Autism Research·Jane YipGene J Blatt
Oct 24, 2006·Biochemical and Biophysical Research Communications·Xiuwu ZhangEverett H Ellinwood
Sep 25, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Maria Luisa LeretJosé Carlos González

Citations

Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Jul 1, 1991·Neuron·M G ErlanderA J Tobin
Nov 15, 1996·Science·L C Katz, C J Shatz
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·H AsadaK Obata
Nov 19, 1997·Trends in Neurosciences·Y Ben-AriJ L Gaiarsa

Related Concepts

Cerebellar Mossy Fibers
Organ Culture Techniques
Neurons
Purkinje Cells
Glutamate Decarboxylase
Immunocytochemistry
Gammalon
Gad2
Mice, Knockout
GAD1 gene

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