Development of the GABA system in organotypic culture of hippocampal and cerebellar slices from a 67-kDa isoform of glutamic acid decarboxylase (GAD67)-deficient mice

Neuroscience Research
F Ji, K Obata


Mice lacking glutamic acid decarboxylase 67kDa (GAD67) are born with much reduced GABA contents but do not survive after birth. To estimate the subsequent development of GAD67-deficient neural tissue, the hippocampal and cerebellar slices were prepared while the mice were alive, and subjected to organotypic culture. Although GABA contents were initially less than 5%, they were markedly increased, and GABA-containing fiber networks were well developed during the culture, indicating that GAD65, the other GAD isoform, largely compensates for the lack of GAD67. Cerebellar Purkinje cells developed similarly in culture.


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Related Concepts

Cerebellar Mossy Fibers
Organ Culture Techniques
Purkinje Cells
Glutamate Decarboxylase
Mice, Knockout
GAD1 gene

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