Development of the VCaP androgen-independent model of prostate cancer

Urologic Oncology
Robert D LobergKenneth J Pienta

Abstract

Prostate epithelial cell growth is dependent on the presence of androgens, and transition of prostate cancer to an androgen-independent phenotype results in a highly aggressive, currently incurable cancer. We have developed a new preclinical model of androgen-independent prostate cancer derived from the VCaP prostate cancer epithelial cell line. VCaP cells were subcutaneously implanted and serially passaged in castrated male severe combined immunodeficient mice. Androgen independence was confirmed by WST-1 (a tetrazolium salt) cell proliferation assay in the absence or presence of dihydrotesterone (1-100 nM). VCaP androgen-sensitive cells responded dose dependently to dihydrotesterone, whereas VCaP androgen-independent cells did not alter their proliferation in response to dihydrotesterone. Gene expression of androgen receptor, B-cell lymphoma-2, prostate cancer antigen 3, prostate acid phosphatase, 6 transmembrane epithelial antigen of the prostate, and survivin was determined by polymerase chain reaction amplification. B-cell lymphoma-2 expression was up regulated in the VCaP androgen-independent lines compared to the VCaP androgen-sensitive, suggesting a possible mechanism of androgen independence. Furthermore, tumor-associa...Continue Reading

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Citations

Jan 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Cale D FahrenholtzKerry L Burnstein
May 7, 2014·Hormones & Cancer·Cynthia C T Sprenger, Stephen R Plymate
Aug 24, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A W TolcherJ S de Bono
Feb 11, 2014·Critical Reviews in Oncology/hematology·Khurum H KhanL Rhoda Molife
Jun 16, 2009·BJU International·Heléne GustavssonJan-Erik Damber
Aug 22, 2007·Neoplasia : an International Journal for Oncology Research·Robert D LobergKenneth J Pienta
Apr 13, 2013·Endocrine-related Cancer·Xinyu WuZoran Culig
Mar 28, 2019·Journal of Molecular Endocrinology·Matias KnuuttilaMatti Poutanen
Jun 12, 2019·International Journal of Molecular Sciences·AbdulFattah Salah Fararjeh, Yen-Nien Liu
Jun 28, 2019·Science Translational Medicine·Ning ZhaoKerry L Burnstein
Jul 23, 2020·Cancer Cell International·Carlos D Cruz-HernándezMauricio Rodríguez-Dorantes
Jan 23, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ziyang YuSteven P Balk
Aug 18, 2021·The Prostate·Michael C HaffnerSrinivasan Yegnasubramanian

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