Development of the VIGS System in the Dioecious Plant Silene latifolia

International Journal of Molecular Sciences
Naoko FujitaKen Komatsu

Abstract

(1) Background: Silene latifolia is a dioecious plant, whose sex is determined by XY-type sex chromosomes. Microbotryum lychnidis-dioicae is a smut fungus that infects S. latifolia plants and causes masculinization in female flowers, as if Microbotryum were acting as a sex-determining gene. Recent large-scale sequencing efforts have promised to provide candidate genes that are involved in the sex determination machinery in plants. These candidate genes are to be analyzed for functional characterization. A virus vector can be a tool for functional gene analyses; (2) Methods: To develop a viral vector system in S. latifolia plants, we selected Apple latent spherical virus (ALSV) as an appropriate virus vector that has a wide host range; (3) Results: Following the optimization of the ALSV inoculation method, S. latifolia plants were infected with ALSV at high rates in the upper leaves. In situ hybridization analysis revealed that ALSV can migrate into the flower meristems in S. latifolia plants. Successful VIGS (virus-induced gene silencing) in S. latifolia plants was demonstrated with knockdown of the phytoene desaturase gene. Finally, the developed method was applied to floral organ genes to evaluate its usability in flowers; (4...Continue Reading

References

May 29, 1992·Cell·D WeigelE M Meyerowitz
Aug 1, 1990·The Plant Cell·V F Irish, I M Sussex
Oct 25, 1988·Nucleic Acids Research·R Höfgen, L Willmitzer
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M H KumagaiL K Grill
Aug 1, 1991·The Plant Cell·E. A. Schultz, G. W. Haughn
Jan 1, 1958·Advances in Genetics·M WESTERGAARD
Jan 13, 2006·Virus Research·Shuichiro TakahashiShigetou Namba
May 15, 2012·The Plant Journal : for Cell and Molecular Biology·Katsutomo SasakiNorihiro Ohtsubo
Dec 15, 2015·Annual Review of Plant Biology·Deborah Charlesworth
Mar 8, 2017·Current Biology : CB·John R Pannell
Apr 22, 2017·The Plant Journal : for Cell and Molecular Biology·Tetsuya ChujoKazuhiro Ishibashi
Dec 3, 2017·G3 : Genes - Genomes - Genetics·Su San TohMichael H Perlin
Apr 16, 2018·New Biotechnology·Vojtech HudzieczekBoris Vyskot
May 22, 2018·Molecular Ecology·Dmitry A Filatov
Jul 18, 2018·BMC Genomics·José Luis Rodríguez LorenzoBoris Vyskot
Aug 15, 2018·Nature Plants·Aline MuyleGabriel A B Marais

❮ Previous
Next ❯

Citations

Jun 28, 2020·Molecular Biotechnology·Ravi Suresh DevaniAnjan Kumar Banerjee
Jun 19, 2021·Applications in Plant Sciences·Shane CareyJocelyn C Hall

❮ Previous
Next ❯

Methods Mentioned

BETA
gene knockdown
Reverse Transcription Polymerase Chain Reaction
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.