Development of three-dimensional tissue engineered bone-oral mucosal composite models

Journal of Materials Science. Materials in Medicine
Thafar AlmelaK Moharamzadeh

Abstract

Tissue engineering of bone and oral mucosa have been extensively studied independently. The aim of this study was to develop and investigate a novel combination of bone and oral mucosa in a single 3D in vitro composite tissue mimicking the natural structure of alveolar bone with an overlying oral mucosa. Rat osteosarcoma (ROS) cells were seeded into a hydroxyapatite/tri-calcium phosphate scaffold and bone constructs were cultured in a spinner bioreactor for 3 months. An engineered oral mucosa was fabricated by air/liquid interface culture of immortalized OKF6/TERET-2 oral keratinocytes on collagen gel-embedded fibroblasts. EOM was incorporated into the engineered bone using a tissue adhesive and further cultured prior to qualitative and quantitative assessments. Presto Blue assay revealed that ROS cells remained vital throughout the experiment. The histological and scanning electron microscope examinations showed that the cells proliferated and densely populated the scaffold construct. Micro computed tomography (micro-CT) scanning revealed an increase in closed porosity and a decrease in open and total porosity at the end of the culture period. Histological examination of bone-oral mucosa model showed a relatively differentiate...Continue Reading

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Citations

Nov 3, 2017·Tissue Engineering. Part C, Methods·Thafar AlmelaKeyvan Moharamzadeh
Mar 7, 2019·Tissue Engineering. Part C, Methods·Kyoko NishiyamaMitsuru Akashi
Dec 4, 2019·Journal of Biomedical Materials Research. Part a·Martin HellerWalburgis Brenner
May 14, 2016·Annals of Biomedical Engineering·Joseph VanderburghScott A Guelcher
Dec 5, 2019·Dentistry Journal·Tahani BinaljadmKeyvan Moharamzadeh
Jul 11, 2020·Dentistry Journal·Emilia BarkerKeyvan Moharamzadeh
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Yuan LiuHui Cheng
Aug 13, 2021·Frontiers in Cell and Developmental Biology·Diana Pereira, Inês Sequeira
Jun 1, 2021·Journal of Tissue Engineering and Regenerative Medicine·Daniela Santos Masson-Meyers, Lobat Tayebi
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Fahimeh TabatabaeiLobat Tayebi

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Methods Mentioned

BETA
xenografts
biopsies
biopsy
FCS
scanning electron microscopy

Software Mentioned

SkyScan CTvol
EURMAT

Related Concepts

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