Development of thyroid-stimulating hormone beta subunit-producing cells in the chicken embryonic pituitary gland

Cells, Tissues, Organs
Kazuaki NakamuraTakafumi Sakai

Abstract

In previous studies, the distribution of thyrotropes in the chicken pituitary gland has been analyzed by immunohistochemistry using heterologous antibodies. In this study, we examined the distribution of thyroid-stimulating hormone beta subunit-immunopositive (TSHbeta-ip) cells and the expression of TSHbeta mRNA in the pituitary glands of chicken embryos by immunohistochemistry using a specific antiserum to the chicken TSHbeta, in situ hybridization and RT-PCR. Immunohistochemical and morphometric analyses revealed that the TSHbeta-ip cells first appeared on embryonic day 10 (E10) in the pituitary gland and were mainly distributed in the cephalic lobe and that the cell density on E20 was almost 4 times greater than that on E10. The chicken TSHbeta-ip cells could be classified into two types based on morphological characteristics: round-shaped cells and club-shaped cells, which have long cytoplasmic processes. In situ hybridization analysis revealed that TSHbeta mRNA-expressing cells were expressed from E9 in the cephalic lobe and that the extent of TSHbeta mRNA-expressing cells coincided with that of TSHbeta-ip cells. RT-PCR also showed that TSHbeta mRNA was expressed from E9 and that Pit-1 mRNA was expressed from E5. These res...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H J SteinfelderF E Wondisford
Nov 1, 1972·Canadian Journal of Physiology and Pharmacology·B A McKeown
Aug 1, 1997·General and Comparative Endocrinology·C C Gregory, T E Porter
Mar 22, 2003·Cells, Tissues, Organs·Fumihiko SasakiKazushige Ogawa

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Citations

Feb 14, 2016·General and Comparative Endocrinology·Yugo WatanabeBert De Groef
Dec 21, 2010·General and Comparative Endocrinology·Laura E EllestadTom E Porter
Oct 12, 2010·Domestic Animal Endocrinology·S V H GrommenB De Groef
Feb 23, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nicole ParkinsonAimee K Ryan

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