Development of tools for the genetic manipulation of Pseudomonas aeruginosa

Journal of Microbiological Methods
Sang-Jin SuhDennis E Ohman

Abstract

To facilitate study of the opportunistic bacterial pathogen Pseudomonas aeruginosa, several genetic tools were developed. These tools include a series of cassettes carrying (a) the minimal sequence for the origin of transfer (oriT) of RP4 plasmid for introducing plasmid into P. aeruginosa via conjugation, (b) a minimal sequence for P. aeruginosa replicon (stabilizing fragment or SF) for maintenance of plasmids in P. aeruginosa, and (c) the transcriptionally non-polar tetracycline resistance gene (TcR) for insertional mutagenesis. Additional genetic constructs include (d) two conjugative and suicide lacZ reporter fusion plasmids for studying gene expression at the transcriptional or translational level, (e) a gentamicin resistant promoter-probing mini-Tn5 lacZ, and (f) a tightly regulated T7 promoter/repressor system to control gene expression in P. aeruginosa.

References

Jan 1, 1984·Molecular & General Genetics : MGG·E T Palva, T J Silhavy
Nov 1, 1983·Reviews of Infectious Diseases·A CrossP Meers
Nov 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Laura Silo-SuhDennis E Ohman

❮ Previous
Next ❯

Citations

Oct 20, 2010·Applied Microbiology and Biotechnology·Aidan L KerrBrett A Neilan
Sep 19, 2012·Current Microbiology·Janice L Paletta, Dennis E Ohman
Dec 22, 2009·Applied and Environmental Microbiology·Isabelle Vallet-GelyBruno Lemaitre
Jun 15, 2007·Infection and Immunity·J Andy SchaberKendra P Rumbaugh
Sep 10, 2013·Canadian Journal of Microbiology·Kathryn E Oliver, Laura Silo-Suh
Mar 2, 2011·Canadian Journal of Microbiology·Jessica M HaginsLaura Silo-Suh
May 24, 2005·BMC Microbiology·Kyoung-Hee Choi, Herbert P Schweizer
Jun 19, 2014·Nature Communications·Coralie FumeauxPatrick H Viollier
Nov 21, 2014·Canadian Journal of Microbiology·Jonathan B DanielsLaura Silo-Suh
Jan 11, 2016·Evolution; International Journal of Organic Evolution·Sara Mitri, Kevin R Foster
Jul 21, 2009·Journal of Microbiological Methods·Laura A Silo-SuhSang-Jin Suh
Jul 28, 2011·Molecular Microbiology·Elizabeth E Wyckoff, Shelley M Payne
Jul 6, 2010·Cellular Microbiology·Isabelle Vallet-GelyBruno Lemaitre
Jan 23, 2010·Microbiology·Jessica M HaginsLaura Silo-Suh
Oct 15, 2014·Infection and Immunity·Jessica A Scoffield, Hui Wu
Jul 9, 2016·Canadian Journal of Microbiology·Jessica Scoffield, Laura Silo-Suh
Jun 20, 2013·The Journal of Biological Chemistry·Laura M RileyP Lynne Howell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.