Development of transgenic rice plants overexpressing the Arabidopsis H+/Ca2+ antiporter CAX1 gene

Plant Cell Reports
K-M KimJ-K Sohn

Abstract

The gene of the Arabidopsis thaliana H+/Ca2+ transporter, CAX1 (cation exchanger 1) was introduced into Japonica cultivars of rice (Ilpumbyeo) by Agrobacterium-mediated transformation, and a large number of transgenic plants were produced. The neomycin phosphotransferase II (NPTII) gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice callus. The introduction of the CAX1 gene was also proven by PCR using CAX1-specific oligonucleotide primers in regenerated plants. Stable integration and expression of the CAX1 gene in T0 plants and T1 progeny were confirmed by DNA hybridization, Northern blot analysis, and luminescent analysis.

References

Nov 1, 1992·The Plant Journal : for Cell and Molecular Biology·M KawaiH Uchimiya
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·K D HirschiG R Fink
Apr 24, 1999·The Plant Cell·D SandersJ F Harper
May 15, 2002·The Journal of Biological Chemistry·Jon K PittmanKendal D Hirschi
Jun 1, 1996·Plant Cell Reports·H RashidK Hinata

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