Development of transmembrane potential in concentric spherical, confocal spheroidal, and bispherical vesicles subjected to nanosecond-pulse electric field

Physical Review. E
Shoubhanik NathRochish M Thaokar

Abstract

Electroporation of concentric compound spherical and confocal spheroidal as well as eccentric compound spherical vesicles, considered to be good models for corresponding nucleate cells, are investigated with an emphasis on their response to nanosecond pulse electric field (nsPEF). Analytical models are developed for the estimation of the transmembrane potential (TMP) across the bilayers of the inner and the outer vesicles and finite-element simulations are also carried out for the eccentric case. Our calculations show that with an increase in the aspect ratio, while the TMP decreases when nsPEF is used, it increases for confocal spheroids when the pulse width is greater than the membrane charging time, leading to fully charged vesicles. Bipolar pulses are shown to effectively control the TMP for a desired time period in the nsPEF regime, and a fast decay of the TMP to zero can be achieved by judicious use of pulse polarity. The external conductivity is found to significantly influence the TMP in nsPEF, unlike millisecond pulses where its effect is insignificant. Additionally the critical electric field required to induce a TMP of 1 V at the inner vesicle is presented for different pulse widths, rise time, as well as membrane ca...Continue Reading

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