Development of transplant vasculopathy in aortic allografts correlates with neointimal smooth muscle cell proliferative capacity and fibrocyte frequency

Atherosclerosis
Geanina OnutaJan-Luuk Hillebrands

Abstract

Transplant vasculopathy consists of neointima formation in graft vasculature resulting from vascular smooth muscle cell recruitment and proliferation. Variation in the severity of vasculopathy has been demonstrated. Genetic predisposition is suggested as a putative cause of this variation, although cellular mechanisms are still unknown. Using a rat aorta transplant model we tested the hypothesis that kinetics of development of transplant vasculopathy are related to neointimal smooth muscle cell proliferative capacity and fibrocyte frequency, the latter being putative neointimal smooth muscle ancestral cells. Aortic allografts were transplanted in Lewis and Brown Norway, as well as MHC-congenic Lewis.1N and Brown Norway.1L recipients. Severity of transplant vasculopathy was quantified 4, 8, 12 and 24 weeks after transplantation. Host-endothelial chimerism, as a reflection of vascular injury, was determined by specific immunofluorescence. Neointimal smooth muscle cell proliferative capacity was determined in vitro and in situ. Fibrocyte frequency and phenotype were determined after in vitro culture by cell counting, immunofluorescence and in situ zymography. Compared to Lewis, Brown Norway recipients developed accelerated transpl...Continue Reading

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Citations

Jul 14, 2010·Cardiovascular Research·Amalia ForteMarilena Cipollaro
Jan 12, 2011·Fibrogenesis & Tissue Repair·Ellen C KeeleyRobert M Strieter
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Mar 19, 2014·Transplantation Reviews·Marieke HogenesRoel de Weger
Nov 17, 2020·Journal of the American Heart Association·Laura E BruijnJan H N Lindeman

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