Development of two species-specific fingerprinting probes for broad computer-assisted epidemiological studies of Candida tropicalis.

Journal of Clinical Microbiology
S JolyD R Soll

Abstract

Candida tropicalis has emerged as the second most frequent colonizing Candida species, and it has been documented in nosocomial infections. To develop an effective fingerprinting system for this species, which is amenable to computer-assisted analyses and which provides information on the genetic relatedness of independent isolates, two DNA fragments, Ct3 (18,000 bp) and Ct14 (20,000 bp), were cloned from a genomic library of Sau3AI partial digestion products. Both probes generate complex Southern blot patterns containing 8 to 20 bands, when hybridized to EcoRI- or EcoRI-HaeIII-digested DNA of independent C. tropicalis isolates. The two probes show no cross-hybridization and are both species specific for C. tropicalis. A comparison of the capacity of the two probes to identify the same strain in different isolates, and differentiate unrelated strains, using computer-assisted computation of similarity coefficients and the genesis of dendrograms, suggests that while Ct14 is more effective in grouping independent isolates, Ct3 is more effective in discriminating small differences in the patterns of highly related isolates and is therefore a more effective probe for determining microevolution within a clonal population and substrai...Continue Reading

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Citations

May 18, 1999·Journal of Dental Research·S R LockhartD R Soll
Sep 6, 2002·Microbiology·Salil A LachkeDavid R Soll
Aug 18, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sophie JolyFayyaz S Sutterwala
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Oct 15, 2003·FEMS Immunology and Medical Microbiology·David R Soll, Claude Pujol
Mar 13, 2019·Antimicrobial Agents and Chemotherapy·Arielle ButtsGlen E Palmer
Jun 12, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sophie JolyDavid R Soll
Apr 2, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C Gil-LamaignereF-M C Müller
Feb 7, 2001·Journal of Clinical Microbiology·L EngerD R Soll
Feb 7, 2001·Clinical Microbiology Reviews·D R Soll
Dec 10, 2003·Current Opinion in Microbiology·Fabienne Malagnac, Philippe Silar

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