Development of type-specific PCR for typing Pneumocystis carinii f. sp. hominis based on nucleotide sequence variations of internal transcribed spacer region of rRNA genes.

Journal of Clinical Microbiology
B JiangC H Lee

Abstract

The nucleotide sequence variations in the internal transcribed spacer region 1 (ITS1) and region 2 (ITS2) of rRNA genes were found to be useful for typing Pneumocystis carinii isolates that infect humans. Two types of ITS1 (A and B) and three types of ITS2 (a, b, and c) sequences have been found, and P. carinii isolates are classified based on sequence types of ITS1 and ITS2 as Ax or Bx (where x may be a, b, or c). Type determination has been achieved by sequencing the ITS regions or by reacting the ITS regions amplified by PCR with type-specific oligonucleotide (TSO) probes. However, TSO typing alone does not work on a specimen from an individual who is infected by more than one strain of P. carinii where different ITS1 types are present in the same specimen. In this study, type-specific PCR assays were developed to supplement TSO typing. Type-specific PCR primers were made so that they differ at their 3' ends by the two nucleotides which distinguish type A from type B of ITS1 plus an additional "A" residue at the extreme 3' ends of the primers. These two primers were paired separately with a general primer which anneals to a region downstream from ITS2 to specifically amplify Ax or Bx. The amplified products were then reacted...Continue Reading

References

Aug 1, 1992·PCR Methods and Applications·R S ChaW G Thilly
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D Y WuR B Wallace
Aug 1, 1995·The Journal of Infectious Diseases·S P KeelyA G Smulian
Mar 1, 1993·Journal of Clinical Microbiology·C H LeeJ W Smith
Nov 1, 1995·The Journal of Eukaryotic Microbiology·S BanerjiA E Wakefield

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Citations

May 1, 2001·Clinical and Diagnostic Laboratory Immunology·J Y HsuehC H Lee
Jan 25, 2007·Journal of Medical Microbiology·Sonia BhardwajArunaloke Chakrabarti
Mar 22, 2002·The Journal of Eukaryotic Microbiology·S R MeshnickL Huang
Nov 28, 2002·International Journal of Medical Microbiology : IJMM·James R Stringer

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