Development of vitamin D3 25-hydroxylase activity in rat liver microsomes

Archives of Biochemistry and Biophysics
M Thierry-PalmerA L Free

Abstract

The ontogeny of vitamin D3 25-hydroxylase activity has been determined in liver microsomes of rat fetuses and neonates. Production of 25-hydroxyvitamin D3 was low (0.11 pmol/g liver/h) 3 days prior to birth. Production rates were 1.2, 2.2, 1.8, and 2.8 pmol/g liver/h on Day 0, Day 2, Day 7, and Day 15, respectively. 25-Hydroxyvitamin D3 production in neonates increased sixfold from Day 15 to Day 22 to a value twice that of the mothers (17.6 pmol/g liver/h compared with 7.3 pmol/g liver/h). Activity in the maternal microsomes was constant (0.22 to 0.30 pmol/mg protein/h) except for the day of parturition (0.54 pmol/mg protein/h) and Day 22 postpartum (0.44 pmol/mg protein/h). A cytosolic factor, present as early as 3 days prior to birth, was required for vitamin D3 25-hydroxylase activity in the fetuses and stimulated the 25-hydroxylase reaction (up to 2.5-fold) in neonates and mothers. The ability of cytosol to prevent degradation of vitamin D3 was also present in the fetal stage. These data suggest that microsomal vitamin D3 25-hydroxylase activity in rat liver microsomes develops slowly and reaches full activity near the weaning stage. Since the cytosolic factor(s) is/are present in the fetal stage, the limiting component in ...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B P HalloranH F DeLuca
Jul 1, 1978·The Journal of Pediatrics·G M ChanJ J Steichen
Sep 20, 1976·Biochemical and Biophysical Research Communications·D L CintiF Bronner
Jan 1, 1985·Biology of the Neonate·J MartialM Gascon-Barré
Nov 1, 1985·Pediatric Research·V PlourdeM Gascon-Barré
Jul 23, 1969·Biochemical and Biophysical Research Communications·M Horsting, H F DeLuca
Nov 1, 1969·The Journal of Clinical Investigation·G PonchonH F DeLuca
Jan 1, 1974·Archives of Biochemistry and Biophysics·M H Bhattacharyya, H F DeLuca
Oct 31, 1980·Biochemical and Biophysical Research Communications·M L Miller, J G Ghazarian
Feb 15, 1983·Archives of Biochemistry and Biophysics·B KabakoffH F DeLuca
Sep 28, 1984·Biochemical and Biophysical Research Communications·I Holmberg
Mar 1, 1984·Pediatric Research·T MarkestadD Aarskog
May 11, 1984·The American Journal of Physiology·M M PikeC S Springer
Sep 1, 1980·Archives of Biochemistry and Biophysics·P S Yoon, H F Deluca
Jan 1, 1981·Pharmacology & Therapeutics·C J McDonald

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Citations

Jun 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·K A Richardson, M Thierry-Palmer

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