PMID: 9178040Apr 1, 1997Paper

Development of vulnerability to hypoxic damage in in vitro hippocampal neurons

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
S Di Loreto, Maurizio Balestrino

Abstract

We investigated the relationship between sensitivity to hypoxia and culture age in in vitro hippocampal neurons. Hypoxia was induced by 24 hr incubation in an oxygen-free environment. Up to 6 days in vitro (DIV) mortality was very low or negligible, with few exceptions. Starting at 7 DIV, significant mortality began to be observed; in the age range 7 10 DIV, mortality of 50% or more was observed in five out of 11 experiments (45%) and average mortality was 51 +/- 15% (mean +/- standard deviation, N = 11). In older (12 18 DIV) cultures, mortality of 50% or more was the rule (13 out of 13 experiments) and average mortality was 83 +/- 16% (mean +/- standard deviation, N = 13). The data could be fitted by a sigmoid line (r = 0.87, P < 10(-6) in which 50% mortality corresponds to 8.6 DIV. The N-methyl-D-aspartate antagonist amino-phosphono-valerate and the nitric oxide synthase inhibitor nitroarginine both provided protection. Degree of protection was comparable for the two compounds, but was not observed in cultures younger than approximately 7 DIV. By contrast exogenous creatine was not protective, at variance with findings from other models. The data represent the first description of how sensitivity to hypoxic damage varies duri...Continue Reading

References

Jan 1, 1990·Annual Review of Neuroscience·D W Choi, S M Rothman
Jun 6, 1986·Neuroscience Letters·P G Aitken, S J Schiff
Nov 1, 1982·The Journal of Physiology·I S Kass, P Lipton
Apr 29, 1983·Science·S M Rothman
Jun 1, 1995·Journal of Neuroscience Methods·M Balestrino
Sep 1, 1994·Brain Research. Molecular Brain Research·M A RivaG Racagni

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Citations

May 18, 2000·Journal of Neuroimmunology·S Di LoretoD Adorno
Dec 3, 2011·Medicinal Research Reviews·Luisa PerassoMaurizio Balestrino
Oct 17, 2012·Neurobiology of Disease·Md Al RahimMir Ahamed Hossain
Nov 10, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J K Newcomb-FernandezR L Hayes
Sep 13, 2000·Molecular Reproduction and Development·J C David, J F Grongnet
May 9, 2002·American Journal of Physiology. Cell Physiology·Junhui ZhangYing Xia
Oct 29, 2019·Journal of Neural Engineering·Lorenzo MuzziJoost le Feber

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