Development of wheat genotypes expressing a glutamine-specific endoprotease from barley and a prolyl endopeptidase from Flavobacterium meningosepticum or Pyrococcus furiosus as a potential remedy to celiac disease

Functional & Integrative Genomics
Claudia OsorioSachin Rustgi

Abstract

Ubiquitous nature of prolamin proteins dubbed gluten from wheat and allied cereals imposes a major challenge in the treatment of celiac disease, an autoimmune disorder with no known treatment other than abstinence diet. Administration of hydrolytic glutenases as food supplement is an alternative to deliver the therapeutic agents directly to the small intestine, where sensitization of immune system and downstream reactions take place. The aim of the present research was to evaluate the capacity of wheat grain to express and store hydrolytic enzymes capable of gluten detoxification. For this purpose, wheat scutellar calli were biolistically transformed to generate plants expressing a combination of glutenase genes for prolamin detoxification. Digestion of prolamins with barley endoprotease B2 (EP-HvB2) combined with Flavobacterium meningosepticum prolyl endopeptidase (PE-FmPep) or Pyrococcus furiosus prolyl endopeptidase (PE-PfuPep) significantly reduced (up to 67%) the amount of the indigestible gluten peptides of all prolamin families tested. Seven of the 168 generated lines showed inheritance of transgene to the T2 generation. Reversed phase high-performance liquid chromatography of gluten extracts under simulated gastrointest...Continue Reading

References

Mar 30, 2001·The Journal of Biological Chemistry·M N HarrisV J Harwood
Jul 4, 2001·European Journal of Gastroenterology & Hepatology·M SternH Wieser
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H QuarstenL M Sollid
Sep 12, 2002·Plant Physiology·M. ChengY. Wan
Sep 28, 2002·Science·Lu ShanChaitan Khosla
Jul 1, 1990·The Plant Cell·W. J. Gordon-KammP. G. Lemaux
Nov 20, 2002·Biological Chemistry·Mónica CappettaLeda Roche
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P A OkubaraT M Hohn
Jun 23, 2004·European Journal of Biochemistry·Jarkko I VenäläinenPekka T Männistö
Feb 16, 2005·Annals of Internal Medicine·Armin Alaedini, Peter H R Green
Jun 2, 2005·Protein Engineering, Design & Selection : PEDS·Norio HamamatsuYasuhiko Shibanaka
Jun 8, 2006·The Journal of Pharmacology and Experimental Therapeutics·Jonathan GassChaitan Khosla
Jun 24, 2006·Chemistry & Biology·Matthew SiegelChaitan Khosla
Dec 13, 2006·Cellular and Molecular Life Sciences : CMLS·J Gass, C Khosla
Mar 14, 2008·Current Protein & Peptide Science·Zoltán Szeltner, László Polgár
Dec 2, 2008·Biological Chemistry·Klaus SchillingBernd Wiederanders
Jul 29, 2009·Scientific American·Alessio Fasano
Nov 28, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Jason A Tye-DinRevati Shreeniwas
Feb 16, 2010·Biotechnology Advances·Priti N DesaiHarish Padh
Mar 16, 2011·Journal of Internal Medicine·L M Sollid, C Khosla
Sep 29, 2011·Digestive Diseases and Sciences·Matthew SiegelDaniel C Adelman
Jan 3, 2012·Methods in Enzymology·Michael T Bethune, Chaitan Khosla
Jan 20, 2012·The American Journal of Clinical Nutrition·Isabel CominoCarolina Sousa
Jun 27, 2012·Functional & Integrative Genomics·C OsorioS Rustgi
Nov 17, 2012·World Journal of Gastroenterology : WJG·Naiyana GujralAlan B R Thomson
Nov 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Shanshan WenSachin Rustgi
Oct 15, 2013·World Journal of Gastroenterology : WJG·Greetje J TackFrits Koning
Mar 4, 2014·Gastroenterology·Marja-Leena LähdeahoMarkku Mäki
Mar 15, 2015·The International Journal of Biochemistry & Cell Biology·Lyudmila V SavvateevaGjumrakch Aliev
May 1, 2015·Digestive Diseases·Katri Kaukinen, Katri Lindfors
Jun 5, 2015·Alimentary Pharmacology & Therapeutics·B N SaldenA A Masclee
Dec 31, 2015·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·A LøvikK E A Lundin
Mar 5, 2016·Journal of Cereal Science·Peter R Shewry, Arthur S Tatham

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Citations

Feb 6, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Daowen WangKunpu Zhang
Jun 14, 2019·Frontiers in Plant Science·Manuel MartinezMercedes Diaz-Mendoza
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Sachin RustgiJan A Delcour
Apr 11, 2021·Applied Microbiology and Biotechnology·Viia Kõiv, Tanel Tenson

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Datasets Mentioned

BETA
M81461
PF0825
U19384

Methods Mentioned

BETA
deamidation
transgenic
PCR
electrophoresis
enzyme-linked
ELISA

Software Mentioned

Sequencher
GeneScript
Visual Basic

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