PMID: 2121605Aug 1, 1990Paper

Development-specific sigma-factor essential for late-stage differentiation of Myxococcus xanthus

Genes & Development
D Apelian, S Inouye

Abstract

The gene for a developmentally expressed sigma-factor, sigB, has been isolated from Myxococcus xanthus by use of the sigA gene (formerly rpoD) of the vegetative sigma-factor as a probe. The sequence of sigB has been determined, and an open reading frame of 193 amino acid residues (Mr = 21,551) was identified. The amino-terminal region of SigB contains 69 residues, of which 35 are identical (50% identity) to the region of SigA required for core RNA polymerase binding and initiation of RNA polymerization. SigB also possesses many features commonly found in other prokaryotic sigma-factors. Analysis of an M. xanthus strain carrying a sigB-lacZ fusion gene revealed that sigB is expressed from a middle to late stage of differentiation corresponding to the period from the onset of sporulation to late development. A sigB deletion mutant displayed normal mound formation and sporulation; however, production of the ops gene product in myxospores of the delta sigB strain was shown to be blocked. Myxospores from the sigB deletion strain also exhibited severe defects in stability and viability during late development. Our data indicate that sigB encodes a sigma-factor essential for the maturation of myxospores at a late stage of M. xanthus d...Continue Reading

References

Jun 1, 1978·Developmental Biology·D C HagenD Kaiser
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Feb 25, 1978·Journal of Molecular Biology·J M CamposD R Zusman
Dec 1, 1988·Journal of Bacteriology·A DhundaleS Inouye
Aug 1, 1989·Journal of Bacteriology·A Rosenbluh, E Rosenberg
Jul 5, 1987·Journal of Molecular Biology·W DonovanR Losick
Sep 1, 1986·Developmental Biology·L KroosD Kaiser
Jan 1, 1986·Annual Review of Genetics·D Kaiser
Jan 1, 1988·Annual Review of Biochemistry·J D Helmann, M J Chamberlin
Oct 1, 1988·Trends in Genetics : TIG·A Ishihama
Sep 1, 1986·Microbiological Reviews·R H Doi, L F Wang
Jan 1, 1986·Annual Review of Genetics·R LosickP J Piggot
Jan 1, 1987·Critical Reviews in Microbiology·L J Shimkets
Sep 1, 1988·Journal of Bacteriology·K A O'Connor, D R Zusman
Mar 1, 1985·Journal of Bacteriology·J S Downard, D R Zusman
Jan 15, 1974·Biochemistry·P Y Chou, G D Fasman
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L Kroos, D Kaiser
Jun 5, 1984·Journal of Molecular Biology·J S DownardD R Zusman
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S InouyeM Inouye
Sep 1, 1981·Cell·R Losick, J Pero
Jul 1, 1982·Journal of Bacteriology·J M Kuner, D Kaiser
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L J ShimketsD Kaiser

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Citations

Nov 1, 1995·Archives of Microbiology·P L Hartzell, P Youderian
Jun 23, 2006·Applied Microbiology and Biotechnology·Toshiyuki Ueki, Sumiko Inouye
Nov 5, 1999·Biochimica Et Biophysica Acta·K YamanakaS Inouye
Dec 3, 2005·Journal of Bacteriology·Toshiyuki UekiSumiko Inouye
Jan 1, 1996·Annual Review of Genetics·C P Wolk
Sep 12, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T Ueki, S Inouye
Jan 1, 1997·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·J E GomezW R Bishai
Jul 20, 2016·Journal of Molecular Biology·Daniel J Bretl, John R Kirby
Nov 18, 1998·FEMS Microbiology Reviews·M M Wösten
Apr 1, 1996·Journal of Bacteriology·A ReisenauerL Shapiro
Jun 1, 1993·Journal of Bacteriology·D Apelian, S Inouye
Aug 1, 1993·Journal of Bacteriology·M Martinez-CanameroS Inouye
Dec 1, 1995·Journal of Bacteriology·W A HanlonS Inouye
Oct 1, 1992·Current Opinion in Genetics & Development·M A Strauch, J A Hoch
Oct 1, 1991·Current Opinion in Genetics & Development·H B Kaplan

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