PMID: 9166111May 1, 1997Paper

Development, validation and application of a two-site enzyme-linked immunosorbent assay for activin-AB

The Journal of Endocrinology
L W EvansN P Groome

Abstract

Monoclonal antibodies, specific for the beta A and beta B subunits of activin, were used to develop a new two-site ELISA for activin-AB. The assay had a detection limit of 0.19 ng/ml. High concentrations of activin-AB were found in bovine, ovine and porcine follicular fluids (FF), with less in human FF (1310, 1730, 688 and 7 ng/ml respectively). Recovery of spiked activin-AB standard from human, bovine and ovine FFs and from homogenized human placental extracts averaged 91%, 115%, 115% and 94% respectively. Within-plate coefficients of variation for different concentration of activin-AB were between 1.3% and 2.67%. The between-plate coefficient of variation was 5.5%. Cross-reactivity experiments showed the high specificity of the assay for activin-AB, with inhibin-A, inhibin-B, follistatin, activin-A and activin-B all cross-reacting < 0.2%. Incubation with high concentrations of follistatin (500 ng/ml) prior to assay did not affect the recovery of activin-AB. Samples of bovine, porcine, ovine and human FF gave dose responses parallel to that of the standard, as did bovine granulosa cell-conditioned media. In human and porcine FF, levels of activin-A and activin-AB were similar whereas, in bovine and ovine FF, activin-A levels w...Continue Reading

Citations

Jun 11, 1998·Molecular Reproduction and Development·J SmitzH Vanderstichele
Jul 14, 2001·Molecular and Cellular Endocrinology·N P GroomeD M Robertson
Jun 15, 2011·Molecular and Cellular Endocrinology·Phil G KnightClaire Glister
Sep 29, 2004·Molecular and Cellular Endocrinology·G M Lambert-MesserlianJ A Canick
May 24, 2015·Molecular and Cellular Endocrinology·Hsun-Ming ChangPeter C K Leung
May 13, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Claire GlisterPhil G Knight
Oct 31, 2009·World Journal of Hepatology·Emanuel KreidlMichael Grusch
Oct 28, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Bassem Refaat, Adil O Bahathiq
Jan 27, 2020·Molecular and Cellular Endocrinology·Beili ChenPeter C K Leung

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