PMID: 20103985Jan 28, 2010Paper

Developmental ability of miniature pig embryos cloned with mesenchymal stem cells

The Journal of Reproduction and Development
Sung-Lim LeeGyu-Jin Rho

Abstract

The present study compared the developmental ability of miniature pig embryos cloned with fetal fibroblasts (FFs), bone marrow-derived mesenchymal stem cells (MSCs) and differentiated (osteocytes, adipocytes and chondrocytes) MSCs. MSCs were isolated from an approximately 1-month-old female miniature pig (T-type, PWG Micro-pig((R)), PWG Genetics Korea). MSCs were differentiated into osteocytes, adipocytes and chondrocytes under controlled conditions and characterized by cell surface antigen profile using specific markers. These differentiated or undifferentiated MSCs, as well as FFs of miniature pig, were transferred into enucleated oocytes of domestic pigs. Data from 10 replicates involving 1567 cloned embryos were assessed in terms of developmental rates. The in vitro development rate to the blastocyst stage of embryos cloned with undifferentiated MSCs was significantly (P<0.05) higher than that of embryos cloned with differentiated MSCs and FFs. Surgical transfer of 523 two-cell stage embryos cloned with undifferentiated MSCs into five synchronized domestic pig recipients resulted in 5 cloned miniature pig offspring (1 stillborn and 4 viable) from 2 pregnant recipients. The results imply that MSCs might be multipotent and th...Continue Reading

References

Jan 1, 1974·The Journal of Investigative Dermatology·L E MillikanP J Manning
Jan 1, 1995·Current Topics in Developmental Biology·F Z Sun, R M Moor
Feb 1, 1997·Journal of Cellular Biochemistry·N JaiswalS P Bruder
Feb 11, 1998·Experimental Cell Research·B JohnstoneJ U Yoo
Jan 20, 1999·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·A G Van DorpM Ponec
Jan 23, 1999·Tissue Engineering·A M MackayM F Pittenger
Apr 2, 1999·Science·M F PittengerD R Marshak
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·T WakayamaP Mombaerts
Feb 2, 2000·Nature Genetics·W M RideoutR Jaenisch
Sep 19, 2000·Nature·I A PolejaevaK H Campbell
Sep 28, 2000·Current Opinion in Immunology·J S Logan
Aug 11, 2001·Science·W M RideoutR Jaenisch
Dec 26, 2001·Biology of Reproduction·Koji YoshiokaShokichi Iwamura
Jan 23, 2002·Blood Purification·Norberto PericoGiuseppe Remuzzi
May 4, 2002·Molecular and Cellular Endocrinology·R Yanagimachi
Dec 31, 2004·Molecular Reproduction and Development·Yang-Sil KimGyu-Jin Rho
Apr 23, 2005·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Katarina Le Blanc, Olle Ringdén
May 10, 2005·Journal of Cellular Physiology·Victor VacantiTechung Lee
Oct 14, 2006·Molecular Reproduction and Development·B Oback, D N Wells
Dec 22, 2006·The International Journal of Developmental Biology·Hai-Feng JinGyu-Jin Rho
Dec 28, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·B Mohana KumarGyu-Jin Rho
Apr 13, 2007·Molecular Reproduction and Development·Kazuchika MiyoshiMitsutoshi Yoshida
Feb 26, 2008·The Journal of Reproduction and Development·Mayuko KuromeHiroshi Nagashima
Jul 26, 2008·World Journal of Urology·Dave ShuklaDarren R Tyson
Jan 16, 2009·Reproduction in Domestic Animals = Zuchthygiene·Y LiX Chen

❮ Previous
Next ❯

Citations

May 29, 2012·Journal of Assisted Reproduction and Genetics·B Mohana KumarGyu Jin Rho
Jul 19, 2012·In Vitro Cellular & Developmental Biology. Animal·Yanli ZhangFeng Wang
Oct 12, 2013·Cytotherapy·Raquel CalloniJoão Antonio Pegas Henriques
Jul 28, 2016·Cellular Reprogramming·Zhenzhen YangYutao Du
Nov 8, 2013·Polish Journal of Veterinary Sciences·J Opiela, M Samiec
Apr 2, 2020·International Journal of Molecular Sciences·Chantel GouveiaMichael S Pepper

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.