Developmental alterations in casein kinase 2 activity during the morphogenesis of quail secondary palate

The Anatomical Record
B M HehnR M Shah

Abstract

During the progression of avian secondary palate morphogenesis, the rate of cell proliferation declines, whereas the production and accumulation of extracellular matrices increases. To investigate the regulation of these events, we examined the quail secondary palate for the activity of casein kinase 2 (CK 2), a pleiotropic serine/threonine second messenger independent enzyme implicated in cell growth and differentiation. Quail palatal shelves were dissected between days 5 and 9 of incubation, which is the period of palate morphogenesis in quail, and prepared either for light microscopic observations or homogenized, cleared by ultracentrifugation, and then subjected to fractionation on a MonoQ column by fast protein liquid chromatography and Western immunoblotting. Histological examination showed that the palatal shelves appeared on day 5 of incubation and approximated by day 8 of incubation. Fractionation of palate extract using a Mono-Q column revealed the presence of a major peak of phosvitin phosphotransferase activity which eluted with 0.5 M NaCl. This activity peak coincided with the presence of a 42 kDa subunit of CK 2 as determined by Western blotting with a CK 2 specific antibody. The CK 2 activity towards phosvitin wa...Continue Reading

References

Jun 1, 1991·Current Opinion in Cell Biology·H Meisner, M P Czech
Dec 16, 1991·Biochemical and Biophysical Research Communications·M J Birnbaum, V C Glover
Dec 1, 1990·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·S L PelechM Daya-Makin
Apr 30, 1990·European Journal of Biochemistry·U MünstermannO G Issinger
Sep 24, 1990·Biochimica Et Biophysica Acta·L A Pinna
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P AckermanN Osheroff
Jan 1, 1987·Current Topics in Developmental Biology·R M Pratt
Jan 1, 1989·Critical Reviews in Toxicology·R M Greene
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·E G KrebsJ Sommercorn
Sep 11, 1987·Cell·T Hunter
Dec 30, 1987·European Journal of Biochemistry·M PerezE Itarte
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J SommercornE G Krebs
Jan 1, 1984·Current Topics in Developmental Biology·L L Brinkley, J Morris-Wiman
Jul 16, 1982·Biochemical and Biophysical Research Communications·R M GreeneM R Lloyd
Jan 1, 1983·The Journal of Experimental Zoology·R M GreeneZ Davidovitch
Jan 1, 1983·Methods in Enzymology·G M Hathaway, J A Traugh
Oct 29, 1984·FEBS Letters·K ProwaldO G Issinger
Nov 1, 1994·The Journal of Experimental Zoology·R M ShahE J Feeley
Mar 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J E Allende, C C Allende
Nov 1, 1993·Molecular and Cellular Biochemistry·D W Litchfield, B Lüscher
Jan 1, 1994·Acta Anatomica·P MestresO G Issinger
Feb 1, 1994·Anti-cancer Drugs·G S Ben-Khaial, R M Shah

❮ Previous
Next ❯

Citations

Jul 11, 2002·American Journal of Physiology. Cell Physiology·Philip HilgardRichard J Stockert
Oct 30, 2001·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·S G Gong, R L Eulenberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.