Developmental and dysmorphogenic effects of glufosinate ammonium on mouse embryos in culture

Teratogenesis, Carcinogenesis, and Mutagenesis
T Watanabe, T Iwase

Abstract

The effects of glufosinate ammonium on embryonic development in mice were examined using whole embryo and micromass cultures of midbrain and limb bud cells. In day 8 embryos cultured for 48 hr, glufosinate caused significant overall embryonic growth retardation and increased embryolethality to 37.5% at 10 micrograms/ml (5.0 x 10(-5) M). All embryos in the treated groups exhibited specific morphological defects including hypoplasia of the prosencephalon (forebrain) (100%) and visceral arches (100%). In day 10 embryos cultured for 24 hr, glufosinate significantly reduced the crown-rump length and the number of somite pairs, and produced a high incidence of morphological defects (84.6%) at 10 micrograms/ml. These embryos were characterized by blister in the lateral head (100%), hypoplasia of prosencephalon (57.1%), and cleft lips (42.9%) at 20 micrograms/ml (10.0 x 10(-5) M). Histological examination of the treated embryos showed numerous cell death (pyknotic debris) present throughout the neuroepithelium in the brain vesicle and neural tube, but did not involve the underlying mesenchyme. In micromass culture, glufosinate inhibited the differentiation of midbrain cells in day 12 embryos with 50% inhibition occurring at 0.55 microg...Continue Reading

Citations

Jul 12, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Gyu Seek RheeKui Lea Park
Sep 25, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D FabianJ Koppel
Mar 3, 2006·Regulatory Toxicology and Pharmacology : RTP·Rolf Schulte-HermannFrank M Sullivan
Jul 18, 2001·Pest Management Science·N J Kutlesa, S Caveney
Sep 10, 2020·Archives of Environmental Contamination and Toxicology·Oluwaseun Olusegun BabalolaJohannes Hannes Van Wyk
Mar 10, 1998·Human & Experimental Toxicology·T Watanabe, T Sano

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