PMID: 4907883Nov 1, 1969Paper

Developmental and regional variations in ribonucleic acid synthesis on cerebral chromatin

The Biochemical Journal
S C Bondy, S Roberts

Abstract

1. Chromatin was prepared from purified nuclei isolated from liver and cerebral regions of the rat. 2. The capacity of these preparations to promote RNA synthesis in the presence of bacterial RNA polymerase was determined. 3. The rate of RNA synthesis on chromatin was normally 12-21% of the rate observed with native DNA, but was markedly stimulated on addition of 200mm-ammonium sulphate. 4. At physiological concentrations (80mug./ml.), the brain-specific S-100 protein inhibited RNA synthesis on DNA and chromatin. 5. Cerebral chromatin from foetal and newborn animals was more active in RNA synthesis than were the analogous preparations from liver. 6. Cerebellar chromatin maintained a high rate of RNA synthesis during brain maturation. In contrast, RNA synthesis on chromatin from other brain regions and liver declined with age of the rat. 7. RNA synthesized on chromatin stimulated amino acid incorporation in an Escherichia coli ribosomal system and hybridized with homologous DNA. 8. RNA synthesized on chromatin from adult cortex or hindbrain hybridized with DNA to a greater extent than that synthesized on cerebellar chromatin. 9. The proportion of RNA formed on cerebral-cortical chromatin that hybridized with DNA increased with a...Continue Reading

Citations

Jan 1, 1980·The International Journal of Neuroscience·R N Walsh
Nov 1, 1973·Journal of Neurochemistry·I Szijan, J A Burdman
Feb 1, 1979·Journal of Neurochemistry·J S BurksS C Bondy
May 1, 1975·Journal of Neurochemistry·S P Banks-Schlegel, T C Johnson
Jan 1, 1978·Behavioral Biology·L Uphouse, R Moore
Apr 1, 1974·Developmental Biology·F ChytilT C Splesberg
Dec 1, 1976·Neurochemical Research·T C Johnson, P K Weck
Sep 1, 1979·Pharmacology, Biochemistry, and Behavior·B Tedeschi, L Uphouse
Oct 1, 1972·Experimental Gerontology·S M Zhelabovskaya, G D Berdyshev

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