Developmental and Subcellular Organization of Single-Cell C₄ Photosynthesis in Bienertia sinuspersici Determined by Large-Scale Proteomics and cDNA Assembly from 454 DNA Sequencing

Journal of Proteome Research
Sascha OffermannKlaas J van Wijk

Abstract

Kranz C4 species strictly depend on separation of primary and secondary carbon fixation reactions in different cell types. In contrast, the single-cell C4 (SCC4) species Bienertia sinuspersici utilizes intracellular compartmentation including two physiologically and biochemically different chloroplast types; however, information on identity, localization, and induction of proteins required for this SCC4 system is currently very limited. In this study, we determined the distribution of photosynthesis-related proteins and the induction of the C4 system during development by label-free proteomics of subcellular fractions and leaves of different developmental stages. This was enabled by inferring a protein sequence database from 454 sequencing of Bienertia cDNAs. Large-scale proteome rearrangements were observed as C4 photosynthesis developed during leaf maturation. The proteomes of the two chloroplasts are different with differential accumulation of linear and cyclic electron transport components, primary and secondary carbon fixation reactions, and a triose-phosphate shuttle that is shared between the two chloroplast types. This differential protein distribution pattern suggests the presence of a mRNA or protein-sorting mechanism...Continue Reading

References

Jan 1, 1989·The Plant Cell·T Nelson, J A Langdale
Jan 1, 1989·Archives of Biochemistry and Biophysics·S P Robinson, A R Portis
Mar 1, 1993·Plant Physiology·A DedonderE Krebbers
Mar 4, 1998·Plant Molecular Biology·R M EwingJ A Langdale
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·N Zhang, A R Portis
Dec 6, 2001·Nature·E V VoznesenskayaG E Edwards
Dec 26, 2001·Bioinformatics·H H Chou, M H Holmes
Sep 5, 2002·The Plant Journal : for Cell and Molecular Biology·Elena V VoznesenskayaGerald E Edwards
Sep 12, 2002·Annual Review of Plant Biology·Robert J Spreitzer, Michael E Salvucci
Sep 1, 1993·Plant Physiology·Z. DaiG. E. Edwards
Mar 5, 2004·The Plant Journal : for Cell and Molecular Biology·Oliver ThimmMark Stitt
Apr 20, 2004·Molecular Biology of the Cell·Yordanka IvanovaDanny J Schnell
May 13, 2004·The Journal of Cell Biology·Matthew D SmithDanny J Schnell
Oct 26, 2005·Photosynthesis Research·Christine A Raines
Nov 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Atsushi TakabayashiFumihiko Sato
Jan 5, 2007·Annual Review of Plant Biology·Toshiharu Shikanai
May 6, 2008·Molecular & Cellular Proteomics : MCP·Wojciech MajeranKlaas J van Wijk
Sep 23, 2008·Plant Physiology·Israel ZelitchThomas P Brutnell
Jan 24, 2009·Trends in Plant Science·Wojciech Majeran, Klaas J van Wijk
Aug 19, 2009·Journal of Proteome Research·Kelly A DoroshenkThomas W Okita
Oct 16, 2009·Plant & Cell Physiology·Irene GranlundWolfgang P Schröder

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Citations

Oct 14, 2016·Journal of Experimental Botany·Ivan JurićNigel J Burroughs
Apr 13, 2017·Frontiers in Plant Science·Maheshi Dassanayake, John C Larkin
Jul 29, 2020·Frontiers in Plant Science·Keith Ka Ki MaiByung-Ho Kang

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