Developmental appearance, antigenic profile, and proliferation of glial cells of the human embryonic spinal cord: an immunocytochemical study using dissociated cultured cells

Glia
F AloisiG Levi

Abstract

We have investigated the time of appearance of the earliest differentiating glial cell types of human spinal cord using a panel of antigenic markers to identify them in cultures from 6- to 9-week-old human embryos. Immunolabeling performed at 14 h in vitro with the O4 mAb, an early oligodendrocyte marker, showed the presence of oligodendrocytes during the 7th week of age. At 8 weeks only a few of the O4+ cells expressed galactocerebroside (GalC), a marker of more differentiated oligodendrocytes. All the O4+ and GalC+ cells were vimentin+ and some of the GalC+ cells were A2B5+, GD3+ and SSEA-1+. During the first week in vitro many of the O4+ cells exhibiting a more immature, bi- or tri-polar morphology incorporated [3H]thymidine into their nuclei. Cells expressing the astrocyte-specific marker GFAP could be first observed at 8 weeks; almost all of these GFAP+ cells, which should correspond to radial glia on the basis of the current literature, were vimentin+, A2B5+, GD3+, and SSEA-1+. At 2 days in vitro incorporation of [3H]thymidine could be shown in a small fraction of these cells. The finding that radial glia and oligodendrocytes expressed similar antigenic features and the additional observation that a small, but consistent ...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E Bottenstein, G H Sato
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G S EisenbarthM Nirenberg
Jun 1, 1985·Journal of Neuroimmunology·S U Kim
Nov 1, 1989·The Journal of Cell Biology·C GodfraindM Dubois-Dalcq
Jul 1, 1988·Brain Research·G A Elder, E O Major
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·F AloisiG Levi
Dec 1, 1986·Brain : a Journal of Neurology·P G Kennedy, J Fok-Seang
Aug 1, 1985·Brain Research·P A Eccleston, D H Silberberg
Jan 1, 1982·Journal of Neuroimmunology·P G Kennedy
Sep 1, 1984·Brain Research·P A Eccleston, D H Silberberg
Dec 1, 1984·Journal of Neuroimmunology·J E GoldmanT N Seyfried
Sep 1, 1984·Archives of Biochemistry and Biophysics·P FredmanV Ginsburg
Oct 24, 1983·Brain Research·N Kasai, R K Yu
Nov 15, 1983·Biochemical and Biophysical Research Communications·S K KunduD M Marcus
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B RanschtW Seifert

❮ Previous
Next ❯

Citations

Feb 19, 2000·Journal of Neuroscience Research·R LiD W Sah
Oct 15, 1994·Journal of Neuroscience Research·J Satoh, S U Kim
Apr 12, 2002·Brain Research. Developmental Brain Research·Conrad A MessamEugene O Major
Jan 18, 2003·Progress in Neurobiology·Martin Stangel, Hans-Peter Hartung
Feb 1, 1997·Journal of Anatomy·A CompstonN Scolding
Aug 1, 1997·Journal of Neurotrauma·E A ZompaC E Hulsebosch
Dec 22, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·N Scolding
Oct 3, 2007·Regenerative Medicine·Robert H Miller, Lianhua Bai
Nov 21, 2002·The Journal of Comparative Neurology·Siobhan S McMahon, Kieran W McDermott
Jan 2, 2010·Gene Expression Patterns : GEP·Katarina VukojevicMirna Saraga-Babic
Mar 8, 2005·Mathematical Biosciences·Ollivier HyrienAndrei Yakovlev
Oct 14, 2014·BioMed Research International·Justin R SiebertDonna J Osterhout
Aug 1, 1997·Developmental Biology·M S Rao, M Mayer-Proschel
Apr 1, 1997·Multiple Sclerosis : Clinical and Laboratory Research·A Compston
Jul 12, 2005·Archives of Suicide Research : Official Journal of the International Academy for Suicide Research·David Lester
Jul 21, 1999·Journal of Neuroscience Research·W E GreverI D Duncan
Feb 12, 1998·Journal of Neuroscience Research·K Murray, M Dubois-Dalcq
Feb 19, 2000·Journal of Neuroscience Research·S C ZhangI D Duncan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.