Developmental block and programmed cell death in Bos indicus embryos: effects of protein supplementation source and developmental kinetics

PloS One
Sheila Merlo GarciaFlávio Vieira Meirelles

Abstract

The aims of this study were to determine if the protein source of the medium influences zebu embryo development and if developmental kinetics, developmental block and programmed cell death are related. The culture medium was supplemented with either fetal calf serum or bovine serum albumin. The embryos were classified as Fast (n = 1,235) or Slow (n = 485) based on the time required to reach the fourth cell cycle (48 h and 90 h post insemination - hpi -, respectively). The Slow group was further separated into two groups: those presenting exactly 4 cells at 48 hpi (Slow/4 cells) and those that reached the fourth cell cycle at 90 hpi (Slow). Blastocyst quality, DNA fragmentation, mitochondrial membrane potential and signs of apoptosis or necrosis were evaluated. The Slow group had higher incidence of developmental block than the Fast group. The embryos supplemented with fetal calf serum had lower quality. DNA fragmentation and mitochondrial membrane potential were absent in embryos at 48 hpi but present at 90 hpi. Early signs of apoptosis were more frequent in the Slow and Slow/4 cell groups than in the Fast group. We concluded that fetal calf serum reduces blastocyst development and quality, but the mechanism appears to be indep...Continue Reading

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Citations

Jan 8, 2016·Theriogenology·Bruno Valente SanchesMarcelo Marcondes Seneda
Jan 30, 2020·Veterinary Medicine and Science·Sarahí Hernández MartínezMaría Del Carmen Navarro Maldonado
May 23, 2020·Tropical Animal Health and Production·Diego F Carrillo-González, Juan G Maldonado-Estrada
Oct 1, 2019·Zygote : the Biology of Gametes and Early Embryos·Kelly AnnesMarcella Pecora Milazzotto

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Methods Mentioned

BETA
FCS
electrophoresis
fluorescence microscopy

Software Mentioned

Car
KS
R
JMP
SAS

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