Developmental changes and molecular weight of immunoreactive glucocorticoid receptor protein in the ovine fetal hypothalamus and pituitary

Biochemical and Biophysical Research Communications
C J Saoud, C E Wood

Abstract

Parturition in the sheep is initiated by increased activity of the hypothalamus-pituitary-adrenal (HPA) axis in the fetus. Late in gestation, ACTH secretion increases because of increased stimulation to the HPA axis and a decreased cortisol negative feedback inhibition of ACTH secretion. We hypothesized that this alteration in cortisol negative feedback efficacy might result from a decrease in glucocorticoid receptor (GR) protein in the hypothalamus or pituitary. Fetal hypothalami and pituitaries were collected from healthy animals (74 days gestation to 4 weeks postnatally) and analyzed by Western blot for immunoreactive GR (iGR) protein. We detected two forms of iGR: a form consistent in molecular weight with the GR, and a form which is approximately half the size of the full-length receptor. We were not able to detect a developmental change in the intensity of immuno-staining for the full-length receptor protein in these tissues. However, the proportion of half-length relative to full-length GR increased significantly with developmental age. Exogenous estrogen, androgen, or tamoxifen treatment did not significantly affect the expression of GR protein. In conclusion, we detected a 97 kD iGR protein corresponding to the full-le...Continue Reading

Citations

Feb 19, 2002·Kidney International·Vicky HantzisMiodrag Dodic
Jan 29, 2011·Reproductive Sciences·Jared WinikorCharles E Wood
Apr 22, 2008·Gene Expression Patterns : GEP·Christine E SchaubCharles E Wood
Dec 4, 2012·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Eleonora RegueiraNora Raquel Ceballos
Feb 8, 2005·Journal of the Society for Gynecologic Investigation·Charles E Wood
Dec 15, 2005·Physiological Genomics·Maureen Keller-WoodCharles E Wood
Oct 16, 2003·American Journal of Physiology. Endocrinology and Metabolism·Andrew J W FletcherDino A Giussani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics
H NarchiN Kulaylat
Journal of the Society for Gynecologic Investigation
S E MannM G Ross
© 2021 Meta ULC. All rights reserved