Developmental changes in DNA methylation and covalent histone modifications of chromatin associated with the epsilon-, gamma-, and beta-globin gene promoters in Papio anubis

Blood Cells, Molecules & Diseases
Donald LavelleJ DeSimone

Abstract

The baboon is a suitable and relevant animal model to study the mechanism of human globin gene switching. This investigation addresses the role of DNA methylation and histone coding in globin gene switching in the baboon, Papio anubis. Bisulfite sequencing and chromatin immunoprecipitation studies were performed in erythroid cells purified from fetuses of varying gestational ages and from adult bone marrow to analyze the manner that changes in DNA methylation of the epsilon-, gamma-, and beta-globin promoters and association of ac-H3, ac-H4, H3-dimeK4, H3-dimeK36, and H3-dimeK79 with the epsilon-, gamma-, and beta-globin promoters occur during development. Changes in DNA methylation of the epsilon- and gamma-globin gene promoters during transitional stages of globin gene switching were consistent with the stochastic model of methylation and a role of DNA methylation in gene silencing. Enrichment of ac-H3, ac-H4, and pol II at the promoters of developmentally active genes was observed, while the pattern of distribution of H3-dimeK4 and H3-dimeK79 suggests that these modifications are found near both currently and formerly active promoters. Enrichment of H3-dimeK36 at the silenced epsilon-globin gene promoter was observed. These ...Continue Reading

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Citations

May 2, 2015·Epigenetics : Official Journal of the DNA Methylation Society·Maria Armila RuizDonald Lavelle
Mar 21, 2019·British Journal of Haematology·Athena Starlard-DavenportBetty S Pace
Jan 21, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bradley McCollJim Vadolas
Feb 6, 2017·The Journal of Biological Chemistry·Yadong WangQuan Zhao
Dec 13, 2019·Royal Society Open Science·Shengxing LiHanyao Zhang
Jul 23, 2021·Experimental Biology and Medicine·Athena Starlard-DavenportBetty S Pace

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