PMID: 6171267Apr 15, 1981Paper

Developmental changes in the composition of polyadenylated RNA isolated from free and membrane-bound polyribosomes of the rat forebrain, analysed by translation in vitro

The Biochemical Journal
C Hall, L Lim

Abstract

Free and membrane-bound polyribosomes were isolated from the rat forebrain during its development. Polyadenylated RNA [poly(A)+ RNA] was isolated from both fractions, by using oligo(dT)-cellulose chromatography, and its composition studied by translating the poly(A)+ RNA in vitro in reticulocyte lysates. Electrophoretic analysis of the translation products showed that both free and membrane-bound polyribosomal poly(A)+ RNA gave many common components, but that there were also distinct differences in the protein composition of the products of the two fractions. Several proteins, of mol.wts. 39 000, 37 000, 31 000, 27 000 and 17 000, appeared to be products predominantly of free polyribosomal poly(A)+ RNA, whereas others, of mol.wt. 47 000, 33 000, 24 000 and 21 000 were specific to the membrane-bound polyribosomal poly(A)+ RNA fraction. More developmental changes were observed in the translational products of the membrane-bound poly(A)+ RNA fraction. Proteins of mol.wts. 33 000 and 21 000, which were predominant components of the translational products of this fraction when isolated from 10-day and older rats, were not present in translational products derived from preparations isolated from 3-day-old rats. The developmental app...Continue Reading

Citations

Feb 1, 1988·The International Journal of Neuroscience·P I Musajev, D A Kuznetsov
Mar 1, 1988·Journal of Neurology, Neurosurgery, and Psychiatry·C W PerrettS A Whatley
Sep 1, 1986·Neurochemical Research·D Gauthier, M R Ven Murthy
Mar 1, 1992·Schizophrenia Research·C W PerrettR M Marchbanks
Jan 1, 1992·Brain Research. Molecular Brain Research·C W PerrettR M Marchbanks

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.