Developmental changes in the expression of Leishmania chagasi gp63 and heat shock protein in a human macrophage cell line.

Infection and Immunity
J A StreitM E Wilson

Abstract

The ability of the protozoan Leishmania chagasi to infect a vertebrate host depends on its ability to survive intracellularly in a mammalian macrophage. Novel patterns of gene expression are probably important for conversion from the extracellular promastigote to the obligate intracellular amastigote parasite form. We found that the human macrophage-like cell line U937 provided an in vitro model of phagocytosis of L. chagasi promastigotes and intracellular conversion to amastigotes, allowing examination of parasite protein and RNA expression. The Leishmania surface protease gp63 assumed three isoforms during stage conversion, and a 64-kDa form of gp63 not present in promastigotes became the most prominent form in amastigotes. gp63 RNAs derived from the three different classes of msp genes (mspS, mspL, and mspC) were also differentially expressed. Infectious promastigotes contained mRNAs from mspS and mspC genes, whereas converting parasites expressed only mspL and mspC mRNAs. Sequence analysis of clones from an amastigote cDNA library confirmed the presence of gp63 mRNAs only from mspL and mspC class genes in tissue-derived amastigotes. Finally, 24 h after phagocytosis, there was a transient increase in the level of hsp70 and h...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1991·Molecular and Biochemical Parasitology·J R WebbW R McMaster
Nov 1, 1991·Molecular and Biochemical Parasitology·P M RaineyA A Pan
Oct 1, 1991·Experimental Parasitology·P S DoyleD M Dwyer
Jan 1, 1990·Molecular and Biochemical Parasitology·T O FrommelW R McMaster
Oct 1, 1990·Experimental Parasitology·T A GlaserA J Mukkada
Apr 1, 1990·Molecular and Biochemical Parasitology·R M Coulson, D F Smith
Jan 1, 1986·Annual Review of Biochemistry·S Lindquist
Oct 25, 1988·Nucleic Acids Research·M G LeeL H Van der Ploeg
Jun 3, 1988·Science·J A Imlay, S Linn
Feb 1, 1988·Experimental Parasitology·R M SmejkalJ G Olenick
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F Lawrence, M Robert-Gero
Jun 21, 1985·Science·L H Van der PloegC R Cantor
Dec 14, 1984·Biochemical and Biophysical Research Communications·K W HunterE G Hayunga
Nov 1, 1994·Infection and Immunity·M E WilsonB E Britigan
Dec 1, 1993·Molecular and Biochemical Parasitology·S C RobertsJ E Donelson
Jun 1, 1993·Microbiological Reviews·E A CraigR J Nelson
Jan 1, 1993·Molecular and Biochemical Parasitology·E Medina-AcostaD G Russell
Jan 1, 1987·Parasitology Today·C Bordier
Apr 1, 1993·Parasitology Today·P A Bates

❮ Previous
Next ❯

Citations

Jun 25, 2003·Antimicrobial Agents and Chemotherapy·Ruth C MatthewsJames Burnie
Jan 28, 2006·FEMS Microbiology Reviews·James P BurnieRuth C Matthews
Dec 25, 2007·Revista Do Instituto De Medicina Tropical De São Paulo·Yeda L NogueiraPaulo M Nakamura
Apr 20, 2011·Cellular Microbiology·Manjusha M KulkarniBradford S McGwire
Jun 16, 2016·Memórias do Instituto Oswaldo Cruz·Bárbara Santoni CodonhoSelma Giorgio
Jan 17, 2003·The Journal of Biological Chemistry·Stephen D Barr, Lashitew Gedamu
Sep 23, 2003·Infection and Immunity·Katherine A PlewesLashitew Gedamu
Jun 26, 2008·PLoS Neglected Tropical Diseases·Nicholas A Ettinger, Mary E Wilson
Jan 16, 2007·Revista Do Instituto De Medicina Tropical De São Paulo·Yeda L NogueiraEunice A B Galati
Oct 18, 2003·Molecular and Biochemical Parasitology·Chaoqun YaoMary E Wilson
Mar 26, 2004·Vaccine·Ruth C Matthews, James P Burnie
Dec 11, 2007·Molecular and Biochemical Parasitology·Chia-Hung Christine HsiaoMary E Wilson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.