Developmental changes in the heavy subunit of neurofilaments in the corpus callosum of the cat

Brain Research. Developmental Brain Research
A Guadano-FerrazG M Innocenti

Abstract

In the corpus callosum of the cat, the heavy subunit of neurofilaments (NFH) can be demonstrated with the monoclonal antibody NE14, as early as P11, not at P3, and only in a few axons. At P18-19 and more markedly at P29, many more callosal axons have become positive to NE14 and this is similar to what is found in the adult. In contrast, callosal axons become positive to the neurofilament antibody SMI-32 only between P29 and P39 and remain positive in the adult. Treatment with alkaline phosphatase prevents axonal staining with NE14, but results in SMI-32 staining of a few callosal axons as early as P11, but not at P3. Between P11 and P19 the number of axons stained with SMI-32 after alkaline phosphatase treatment increases, in parallel with that of axons stained with NE14. Thus NE14 appears to recognize a phosphorylated form of NFH, while SMI-32 appears to recognize an epitope of NFH which is either masked by phosphate or inaccessible until between P29 and P39, unless the tissue is treated with alkaline phosphatase. These two forms of NFH appear towards the end of the period of massive developmental elimination of callosal axons. They are also synchronous with changes in the spacing of neurofilaments quantified in a separate ult...Continue Reading

References

Jan 1, 1985·Annals of the New York Academy of Sciences·M A Glicksman, M Willard
Jul 1, 1988·Trends in Neurosciences·A Matus
Jan 1, 1986·Journal of Neuroscience Research·D DahlA Bignami
Oct 1, 1988·The Journal of Comparative Neurology·P Berbel, G M Innocenti
Feb 1, 1988·Journal of Neurocytology·R L PriceM J Katz
Apr 1, 1984·The Journal of Cell Biology·N HirokawaM B Willard
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L A Sternberger, N H Sternberger
Feb 10, 1982·The Journal of Comparative Neurology·T Reh, K Kalil

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Citations

Oct 20, 1994·Behavioural Brain Research·G M Innocenti
Oct 20, 1994·Behavioural Brain Research·P BerbelJ Ramón Cerezo
Jan 1, 1994·Brain Research Bulletin·B M Riederer, L I Binder
Sep 26, 2002·Journal of Chemical Neuroanatomy·M T K KirkcaldieJ C Vickers
Oct 1, 1991·The European Journal of Neuroscience·Beat M. Riederer, Giorgio M. Innocenti
Jan 1, 1992·The European Journal of Neuroscience·Beat M. Riederer, Giorgio M. Innocenti
Nov 1, 1993·The European Journal of Neuroscience·P BerbelG M Innocenti
May 29, 2016·Journal of Chemical Neuroanatomy·Matthew T K Kirkcaldie, Jessica M Collins
Oct 12, 1999·The Journal of Comparative Neurology·J P Hornung, B M Riederer
Jan 22, 1996·The Journal of Comparative Neurology·B M RiedererR A Marugg

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