Developmental changes of Ni(2+) sensitivity and automaticity in Nkx2.5-positive cardiac precursor cells from murine embryonic stem cell

Circulation Journal : Official Journal of the Japanese Circulation Society
Kasumi ManabeIchiro Hisatome

Abstract

It is controversial which subtypes of T type Ca(2+) channels are implicated in automaticity of cardiac cells during the embryonic period. The effect of Ni(2+) on the automaticity of Nkx2.5-positive cardiac precursor cells sorted from embryonic stem cells during their differentiation was examined using patch clamp techniques. Although 40 micromol/L Ni(2+), which is enough to block Ni(2+)sensitive T type-Ca(2+) channels, decreased the spontaneous beating rate in all cells in the early and intermediate stage, Ni(2+) did not show any effects on the automaticity of 50% of the cells in the late stage. These results indicate that Ni(2+)-sensitive T-type Ca(2+) channels expressed in the Nkx2.5-positive cardiac precursor cells are developmentally regulated.

References

Jul 7, 2000·Developmental Biology·V M ChristoffelsA F Moorman
Jun 21, 2002·Circulation Journal : Official Journal of the Japanese Circulation Society·Yuichi IkedaIssei Komuro
Sep 13, 2002·Circulation Journal : Official Journal of the Japanese Circulation Society·Toru YatsuhashiChiaki Shigemasa
Feb 21, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kyoko HidakaTakayuki Morisaki
Aug 16, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Ying Ming ZhangSamuel C Dudley
Dec 26, 2003·Circulation Journal : Official Journal of the Japanese Circulation Society·Eishun HoribeHisayoshi Fujiwara
Feb 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Noriko NiwaItsuo Kodama

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Citations

Oct 1, 2005·Circulation Journal : Official Journal of the Japanese Circulation Society·Einosuke MizutaIchiro Hisatome
Oct 26, 2005·Circulation Journal : Official Journal of the Japanese Circulation Society·Haruki TakemuraItsuo Kodama
May 15, 2007·Journal of Molecular and Cellular Cardiology·Yan WangKatsushige Ono

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