Developmental characteristics of adapting mouse small intestine crypt cells

Gastroenterology
C R ErwinMichael D Bates

Abstract

Following massive small bowel resection (SBR), the remnant intestine undergoes an adaptive process characterized by increases in a number of physiologic and morphologic parameters. These changes are the result of a stimulus that increases crypt cell mitosis and augments cellular progression along the villus axis. To better define this process, we identified patterns of gene expression specifically within adapting intestinal crypt cells following SBR. Laser capture microdissection was used to isolate mouse intestinal crypt cells following SBR or sham operation. Multiple biological and technical complementary DNA microarray replicates allowed rigorous statistical analyses for identification of important expression profiles. Major groups of genes were classified as to site of action, functional pathway, and possible regulatory groups. A total of 300 genes differentially expressed at significant levels within adapting crypt enterocytes were analyzed. Comparison of this list of differentially expressed adapting crypt cell genes with a generalized mouse gene expression database (from 82 developing and adult mouse tissues) showed the greatest overlap with developing and immature intestinal tissues. We identified prominent groups of ge...Continue Reading

Citations

Jul 26, 2008·Development·Michael J GeskeThaddeus S Stappenbeck
Sep 1, 2012·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·Chirag Sureshchandra DesaiThomas M Fishbein
Apr 25, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Francisca JolyMuriel Thomas
Jun 15, 2011·Alimentary Pharmacology & Therapeutics·R A Hejazi, R W McCallum
Jul 24, 2012·Journal of Pediatric Gastroenterology and Nutrition·Vittoria BuccigrossiAlfredo Guarino
Dec 15, 2020·Current Treatment Options in Pediatrics·Baddr A Shakhsheer, Brad W Warner
Aug 18, 2010·Journal of Pediatric Surgery·Kate L HealeyPrue M Pereira-Fantini

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