Developmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue

Zygote : the Biology of Gametes and Early Embryos
Gustavo Bruno MotaLuiz Sergio de Almeida Camargo

Abstract

The objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus-oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 degrees C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB- (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB- group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB- (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB- (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ grou...Continue Reading

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Citations

Aug 27, 2011·Journal of Assisted Reproduction and Genetics·Abdollah Mohammadi-SangcheshmehMichael Hoelker
Jul 28, 2011·Zygote : the Biology of Gametes and Early Embryos·Diego Duarte AlcobaAlexandre Tavares Duarte de Oliveira
Dec 21, 2013·Journal of Assisted Reproduction and Genetics·Amir Hossein Abazari-KiaMohammad Salehi
Apr 30, 2015·Zygote : the Biology of Gametes and Early Embryos·Diego Duarte AlcobaIlma Simoni Brum
Aug 10, 2012·Reproductive Medicine and Biology·Hiroaki Funahashi

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