Developmental competence of 8?16-cell stage bison embryos produced by interspecies somatic cell nuclear transfer

Reproduction, Fertility, and Development
L Antonio González-GrajalesGabriela F Mastromonaco

Abstract

Altered communication between nuclear and cytoplasmic components has been linked to impaired development in interspecies somatic cell nuclear transfer (iSCNT) embryos as a result of genetic divergence between the two species. This study investigated the developmental potential and mitochondrial function of cattle (Bos taurus), plains bison (Bison bison bison) and wood bison (Bison bison athabascae) embryos produced by iSCNT using domestic cattle oocytes as cytoplasts. Embryos in all groups were analysed for development, accumulation of ATP, apoptosis and gene expression of nuclear- and mitochondrial-encoded genes at the 8-16-cell stage. The results of this study showed no significant differences in the proportion of developed embryos at the 2-, 4- and 8-16-cell stages between groups. However, significantly higher ATP levels were observed in cattle SCNT embryos compared with bison iSCNT embryos. Significantly more condensed and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL)-positive nuclei were found in plains bison iSCNT embryos. No significant differences in the expression levels of nuclear respiratory factor 2 (NRF2) or mitochondrial subunit 2 of cytochrome c oxidase (mt-COX2) were found in any...Continue Reading

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Citations

Nov 29, 2017·Reproduction, Fertility, and Development·N-Y RhoL A Favetta

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Methods Mentioned

BETA
light microscopy
biopsy
Assay
PCR

Software Mentioned

Primer
CFX Manager
iSCNT
RSTUDIO
BLAST

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