PMID: 9657970Jul 11, 1998Paper

Developmental effect of polyamine depletion in Caenorhabditis elegans

The Biochemical Journal
M MacraeP Coffino

Abstract

Ornithine decarboxylase (ODC) catalyses the conversion of ornithine to putrescine, an obligate precursor to the polyamines spermidine and spermine. We reported previously that homozygous odc-1 (pc13) worms have no detectable ODC activity. Despite their inability to make polyamines, these mutant worms appear normal, but with a slight reduction in total brood size, when grown in complex medium that presumably contains polyamines. We now show that when ODC-deficient worms are transferred to polyamine-free medium, they show a strong phenotype. odc-1 worms have two different fates, depending upon the developmental stage at which polyamines are removed. If the polyamines are removed at the L1 larval stage, the mutant animals develop into adult hermaphrodites that produce very few or no eggs. In contrast, if mutant larvae at the later L4 stage of development are transferred to polyamine-deficient medium, they develop and lay eggs normally. However, approx. 90% of the eggs yield embryos that, although well differentiated, arrest at early stage 3. Either maternal or zygotic expression of ODC provides partial rescue of embryonic lethality. Supplementing deficient medium with the polyamine spermidine allows ODC-deficient worms to develop ...Continue Reading

Citations

Mar 11, 2004·European Journal of Biochemistry·Juhani JänneTuomo A Keinänen
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·P Coffino
Oct 15, 2005·IUBMB Life·Ursula Mangold
Sep 19, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexander HeinickKai Lüersen
Mar 18, 2011·Journal of Cellular Physiology·Nicole K L Lee, Helen E MacLean
Mar 10, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A Gritli-LindeA Linde
Sep 5, 2001·Molecular and Cellular Biology·H PendevilleJ L Cleveland
Apr 22, 2006·International Journal of Systematic and Evolutionary Microbiology·Mohammad Abdul BakirYoshimi Benno
Apr 21, 2011·American Journal of Physiology. Endocrinology and Metabolism·Nicole K L LeeHelen E MacLean
Jun 3, 2015·The Journal of Biological Chemistry·Dirk StegehakeEva Liebau

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