Developmental expression and localization of the catalytic subunit of protein phosphatase 2A in rat lung

Developmental Dynamics : an Official Publication of the American Association of Anatomists
C XueA D Everett

Abstract

Protein phosphatase type-2A (PP2A) is a highly conserved serine/threonine phosphatase known to play a key role in cell proliferation and differentiation in vitro, but the role of PP2A in mammalian embryogenesis remains unexplored. No particular information exists as to the tissue or cell specific expression of PP2A or the relevance of PP2A expression to mammalian development in vivo. To examine expression of PP2A during mammalian lung development, we studied fetal rats from day 14 of gestation (the lung bud is formed on day 12 of gestation) to parturition. Western analysis with a specific PP2A catalytic subunit antibody identified a single 36 kDa protein, with protein levels two-fold higher in the 17 and 19 day embryonic lung as compared to the adult. With in situ hybridization and immunohistochemistry, both mRNA and protein for PP2A were localized equally to the epithelial lining of the embryonic lung airway and the surrounding mesenchyme in the 14 day embryonic lung. With maturation of the lung, PP2A becomes highly expressed in respiratory epithelium. The highest level of expression was in the earliest developing airways with columnar epithelium (the pseudoglandular stage, 15-18 days of gestation). There was a decrease in exp...Continue Reading

References

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Citations

Apr 5, 2005·Toxicon : Official Journal of the International Society on Toxinology·Janet M BensonLora E Fleming
Apr 27, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·B K TaylorA D Everett
Nov 18, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Miyoung YoonHugh A Barton
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Allen D EverettDavid L Brautigan
Aug 14, 1999·Journal of the American Society of Nephrology : JASN·A D EverettT Stoops

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