Developmental expression and substrate specificities of alfalfa caffeic acid 3-O-methyltransferase and caffeoyl coenzyme A 3-O-methyltransferase in relation to lignification

Plant Physiology
K InoueR A Dixon

Abstract

The biosynthesis of monolignols can potentially occur via two parallel pathways involving free acids or their coenzyme A (CoA) esters. Caffeic acid 3-O-methyltransferase (COMT) and caffeoyl CoA 3-O-methyltransferase (CCOMT) catalyze functionally identical reactions in these two pathways, resulting in the formation of mono- or dimethoxylated lignin precursors. The activities of the two enzymes increase from the first to the sixth internode in stems of alfalfa (Medicago sativa L.), preceding the deposition of lignin. Alfalfa CCOMT is highly similar at the amino acid sequence level to the CCOMT from parsley, although it contains a six-amino acid insertion near the N terminus. Transcripts encoding both COMT and CCOMT are primarily localized to vascular tissue in alfalfa stems. Alfalfa CCOMT expressed in Escherichia coli catalyzes O-methylation of caffeoyl and 5-hydroxyferuloyl CoA, with preference for caffeoyl CoA. It has low activity against the free acids. COMT expressed in E. coli is active against both caffeic and 5-hydroxyferulic acids, with preference for the latter compound. Surprisingly, very little extractable O-methyltransferase activity versus 5-hydroxyferuloyl CoA is present in alfalfa stem internodes, in which relative...Continue Reading

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Citations

Jun 30, 2001·Phytochemistry·Z H YeD S Himmelsbach
Jan 5, 2002·Phytochemistry·Gudrun SchröderJoachim Schröder
Apr 23, 2002·The Plant Journal : for Cell and Molecular Biology·Rochus FrankeClint Chapple
Mar 1, 2005·Plant Physiology·Jean-Luc FerrerJoseph P Noel
Dec 7, 2007·Genome Génome / Conseil National De Recherches Canada·Kevin C LamSelvadurai Dayanandan
Oct 20, 2005·Journal of Bioscience and Bioengineering·Shuiqin WuYutaka Ebizuka
Feb 3, 2000·Molecular Plant-microbe Interactions : MPMI·J I Jiménez-ZurdoA Kondorosi
May 5, 2000·Molecular Plant-microbe Interactions : MPMI·J D Hipskind, N L Paiva
Feb 7, 2016·Plant Physiology and Biochemistry : PPB·Debora GiordanoAndrea Schubert
Jul 31, 2012·Journal of Plant Physiology·Yun WangStephen Chivasa
Feb 15, 2008·Plant Physiology and Biochemistry : PPB·J-L FerrerJ P Noel
Mar 18, 2008·Journal of Molecular Biology·Jakub G KopyckiMilton T Stubbs
May 31, 2017·Plant, Cell & Environment·Weronika CzarnockaStanisław Karpiński
Feb 7, 2018·Journal of the Science of Food and Agriculture·Girma GetachewEdward J DePeters

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