Developmental expression of a functional TASK-1 2P domain K+ channel in embryonic chick heart.

Journal of Biomedical Science
Hengtao ZhangTony L Creazzo

Abstract

Background K+ channels are the principal determinants of the resting membrane potential (RMP) in cardiac myocytes and thus, influence the magnitude and time course of the action potential (AP). RT-PCR and in situ hybridization are used to study the distribution of TASK-1 and whole-cell patch clamp technique is employed to determine the functional expression of TASK-1 in embryonic chick heart. Chicken TASK-1 was expressed in the early tubular heart, then substantially decreased in the ventricles by embryonic day 5 (ED5), but remained relatively high in ED5 and ED11 atria. Unlike TASK-1, TASK-3 was uniformly expressed in heart at all developmental stages. In situ hybridization studies further revealed that TASK-1 was expressed throughout myocardium at Hamilton-Hamburger stages 11 and 18 (S11 & S18) heart. In ED11 heart, TASK-1 expression was more restricted to atria. Consistent with TASK-1 expression data, patch clamp studies indicated that there was little TASK-1 current, as measured by the difference currents between pH 8.4 and pH 7.4, in ED5 and ED11 ventricular myocytes. However, TASK-1 current was present in the early embryonic heart and ED11 atrial myocytes. TASK-1 currents were also identified as 3 microM anandamide-sensit...Continue Reading

References

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Citations

Nov 19, 2014·Pflügers Archiv : European journal of physiology·Niels DecherSusanne Rinné

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Methods Mentioned

BETA
PCR
dissection
enzymatic dissociation

Software Mentioned

Axon Clampex
pCLAMP

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