Developmental expression of a Trypanosoma cruzi phosphoinositide-specific phospholipase C in amastigotes and stimulation of host phosphoinositide hydrolysis.

Infection and Immunity
Vicente de Paulo MartinsS N Moreno

Abstract

Phosphoinositide phospholipase C (PI-PLC) plays an essential role in cell signaling. A unique Trypanosoma cruzi PI-PLC (TcPI-PLC) is lipid modified in its N terminus and localizes to the outer surface of the plasma membrane of amastigotes. We show here that TcPI-PLC is developmentally regulated in amastigotes and shows two peaks of surface expression during the developmental cycle of T. cruzi, the first immediately after differentiation of trypomastigotes into amastigotes and the second before differentiation of amastigotes into trypomastigotes. Surface expression of TcPI-PLC coincides with phosphatidylinositol 4,5-bisphosphate (PIP(2)) depletion in the host cell membrane and with an increase in the levels of its product, inositol 1,4,5-trisphosphate. During extracellular differentiation, PI-PLC is secreted into the incubation medium. Maximal early expression of TcPI-PLC on the surface of amastigotes and PIP(2) depletion coincide with host cytoskeletal changes, Ca(2+) signaling, and transcriptional responses described previously. The presence of TcPI-PLC on the outer surface of the plasma membrane of the parasite and the capacity to be secreted and to alter host phospholipids are novel mechanisms of the host-parasite interaction.

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Citations

Jul 3, 2013·Microbes and Infection·Abdur RubYusuf Akhter
Jul 22, 2010·The Journal of Biological Chemistry·Vicente de Paulo MartinsSilvia N J Moreno
Dec 3, 2013·European Journal of Pharmacology·Roberto DocampoHelmut Plattner
Sep 26, 2012·Microbes and Infection·Luis OsorioJorge González
Feb 26, 2011·Immunological Reviews·L David Sibley
Nov 26, 2015·IUBMB Life·Scott M LandfearMarco A Sanchez
May 4, 2010·PLoS Neglected Tropical Diseases·Christopher Kribs-Zaleta
Apr 11, 2020·PLoS Neglected Tropical Diseases·Alfredo MijaresJosé R Lopez
Feb 22, 2011·The Journal of Eukaryotic Microbiology·Rosa M De LederkremerRoberto Docampo
Apr 9, 2016·PLoS Pathogens·Andrés B LantosOscar Campetella
Jan 10, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Roberto Docampo, Guozhong Huang
Sep 13, 2019·Acta Tropica·Lizette E RiosNisha J Garg
May 14, 2021·AMB Express·Hamed Memariani, Mojtaba Memariani

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