Developmental expression of a type II collagen/beta-galactosidase fusion gene in transgenic mice

Developmental Dynamics : an Official Publication of the American Association of Anatomists
M MetsarantaE Vuorio

Abstract

The correct temporal and spatial expression of the type II collagen gene is believed to be important for normal development and growth of the skeleton and the eye, i.e., tissues where the protein product is predominantly found. To study transcriptional activation of type II collagen gene in skeletal and nonskeletal tissues we produced transgenic mice carrying murine proalpha1(II) collagen/beta-galactosidase fusion gene constructs. The expression of the fusion gene was found to depend on the presence of intron 1 deleted failed to reveal any beta-galactosidase activity confirming the important role of regulatory sequences within intron 1 of the gene. High-level expression of the functional construct was clearly confined to cartilaginous tissues but transient low-level expression was also observed in extraskeletal locations, such as the developing brain and the notochord. The results demonstrate that the regulatory elements in the proalpha1(II) collagen/beta-galactosidase fusion gene construct confer both temporal and spatial specificity indistinguishable from that of the endogenous proalpha1(II) collagen gene as determined by the presence of the corresponding mRNA by in situ hybridization. Furthermore the beta-galactosidase activ...Continue Reading

References

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Citations

Feb 27, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·K SakaiA Aszódi
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·E C WeirA E Broadus
Jan 13, 2000·Immunological Reviews·G SønderstrupH O McDevitt
Dec 23, 2011·Development·Weiguang WangXiangli Yang
Nov 26, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S GarofaloA Yayon
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·M YamaguchiA Kakizuka
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·W M PhilbrickA C Karaplis
Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T D GrantW A Horton
Feb 22, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Marianna A TryfonidouWilliam A Horton

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