Developmental expression of endothelin receptors in postnatal swine mesenteric artery

Pediatric Research
Baogen Y SuCraig A Nankervis

Abstract

Studies were conducted to determine whether endothelin (ET) ETA and ETB receptor protein and mRNA expression is developmentally regulated in the postnatal swine mesenteric circulation. To this end, Western blotting and real-time reverse PCR were performed on protein and total RNA isolated from the mesenteric artery harvested from 3-, 10-, and 30-d-old swine. Western blot analysis revealed that ETA and ETB receptor protein expression in the swine mesenteric artery decreased over the age range studied; thus, ETA and ETB receptor protein expression was significantly greater in the 3-d-old group then progressively declined over the first postnatal month. Similar to the Western data, real-time PCR analysis revealed that ETA and ETB receptor mRNA expression also decreased over the age range studied; thus, ETA and ETB receptor mRNA expression was significantly greater in the 3-d-old group then progressively declined over the first postnatal month. Immunohistochemistry localized the ETA receptor to the vascular smooth muscle and the ETB receptor to the endothelial cell layer. Additionally, we report a partial cDNA sequence for the swine ETB receptor. We conclude that ETA and ETB receptor protein and mRNA expression is developmentally r...Continue Reading

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Citations

Aug 21, 2010·Canadian Journal of Physiology and Pharmacology·Levon AvedanianDanielle Jacques
Apr 25, 2013·Seminars in Pediatric Surgery·Daniel J Watkins, Gail E Besner
Mar 19, 2008·Seminars in Perinatology·Craig A NankervisKristina M Reber
Dec 26, 2006·The Journal of Pediatrics·Philip T NowickiCraig A Nankervis
Jun 20, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Philip T Nowicki
Jan 15, 2013·Physical & Occupational Therapy in Pediatrics·Eilish Byrne, June Garber
Jul 29, 2020·Frontiers in Pediatrics·Baukje M DotingaElisabeth M W Kooi

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