Abstract
The molecular techniques of in situ transcription and antisense RNA amplification (IST/aRNA) have allowed for the monitoring of coordinate changes in the expression of multiple genes simultaneously. However, the analysis of their concurrent behavior during murine embryogenesis has been problematic. Studies involving the investigation of temporal and spatial gene expression during embryogenesis have focused solely on the analysis of isolated, single gene events. Such an approach has failed to provide an integrative picture of genetic control over the varied and complicated cellular processes governing embryogenesis. In order to interpret the enormous amount of gene expression data generated by these procedures, we have attempted to develop an analytical framework by employing the statistical concepts of principal components analysis (PCA). For the current study, we performed IST/aRNA on neural tubes dissected from the highly inbred LM/Bc murine strain collected during four gestational time periods. A subset of these genes, representing a partial signaling pathway in the developing neuroepithelium, was then subjected to PCA. Here, we report that PCA highlighted the transcriptional interplay among the genes p53, wee-1, Tgf beta-2,...Continue Reading
References
Dec 11, 1992·Cell·J L WranaJ Massagué
Dec 11, 1992·Cell·D L Stemple, D J Anderson
Dec 18, 1992·Science·D L VauxS K Kim
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J EberwineP Coleman
Sep 5, 1991·Nature·M IgarashiH Okayama
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W E MercerS J Ullrich
Nov 1, 1990·Molecular and Cellular Biology·L DillerB Vogelstein
Sep 29, 1988·Nature·D L VauxJ M Adams
Sep 1, 1967·The Journal of Comparative Neurology·J Langman, G W Welch
Oct 1, 1984·Teratology·J Bannigan, D Cottell
Aug 1, 1980·Teratology·W A Cole, D G Trasler
Jun 1, 1995·Nature Genetics·V P SahT Jacks
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y Geng, R A Weinberg
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·J H PrehnR J Miller
Jan 15, 1995·Genes & Development·M E EwenD S Peeper
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·J J RyanM F Clarke
Mar 15, 1994·International Journal of Cancer. Journal International Du Cancer·T FujiwaraE A Grimm
Jan 1, 1994·The Journal of Cell Biology·J C Reed
May 28, 1993·Science·R H ChenR Derynck
May 28, 1993·Science·R EbnerR Derynck
Apr 29, 1993·Nature·A R ClarkeA H Wyllie
Nov 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R M Elledge, W H Lee
Oct 1, 1995·Molecular Biology of the Cell·S Davey, D Beach
Jan 1, 1996·Developmental Genetics·B WlodraczykR H Finnell
Citations
Mar 28, 1998·Teratology·G D BennettR H Finnell
Feb 9, 2000·Trends in Pharmacological Sciences·A K DixonK Lee
Aug 30, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Milena NasiAndrea Cossarizza
Nov 5, 1999·Alcoholism, Clinical and Experimental Research·R E McAlhanyJ R West
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·N ChowP D Coleman
Jun 26, 2018·Biomicrofluidics·M HerbigO Otto
Feb 26, 2000·Reproductive Toxicology·G D BennettR H Finnell
Oct 28, 1999·Brain Research·L R KisleyS J Fluharty
Mar 25, 2004·Reproductive Biomedicine Online·Mercedes G BermúdezNury M Steuerwald