PMID: 8943024Nov 26, 1996Paper

Developmental expression of synthetic cis-regulatory systems composed of spatial control elements from two different genes

Proceedings of the National Academy of Sciences of the United States of America
C V KirchhamerE H Davidson

Abstract

Synthetic cis-regulatory systems consisting of positively and negatively acting cis-regulatory modules of the Endo16 gene were combined with the lineage-specific regulatory element of the SM50 gene associated with a reporter and injected into eggs of sea urchins. We show here that synthetic cis-regulatory systems consisting of the positive Endo16 regulatory elements linked with the SM50 regulatory element are expressed spatially exactly as the sum of the individual endodermal and skeletogenic expression patterns. In combination, both lineage-specific positive regulatory elements function autonomously. However, addition of the Endo16 regulatory module that represses ectopic skeletogenic expression of Endo16 receptor constructs does not affect expression driven by the SM50 regulatory elements in the same skeletogenic cells. The repression function of this element is thus dedicated to control of the positive spatial output of the Endo16 regulatory system.

References

Jun 1, 1987·Developmental Biology·B R Hough-EvansE H Davidson
Jun 1, 1971·The Quarterly Review of Biology·R J Britten, E H Davidson
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A P McMahonE H Davidson
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·H N CaiM Levine
Sep 27, 2012·Molecular Systems Biology·Michael A WhiteBarak A Cohen

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Citations

Jul 14, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G M Wessel, A Wikramanayake
Nov 21, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G Dover
Mar 10, 2001·Cell Biology International·J P HapgoodS D Scherer
May 25, 2002·Developmental Biology·Paola OliveriEric H Davidson
Mar 10, 2009·Genomics·Axel ViselLen A Pennacchio
Jun 25, 2002·Journal of Theoretical Biology·Olivier Cinquin, Jacques Demongeot
Jan 24, 2012·Gene Regulation and Systems Biology·Jasmine KharazmiThomas Brody

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