PMID: 2120112Jul 1, 1990Paper

Developmental expression of the endogenous TIMP gene and a TIMP-lacZ fusion gene in transgenic mice

Genes & Development
A M Flenniken, B R Williams

Abstract

We determined the expression pattern of the tissue inhibitor of metalloproteinase (TIMP) in the development of the mouse embryo using in situ hybridization and transgenesis. Localized TIMP RNA was first detected at 13.5 days post conceptus (p.c.) in tissues undergoing osteogenesis, such as the mandible, ribs, and calvaria. As development proceeded, TIMP RNA could be detected at additional sites, including the tooth buds, vertebrae, and long bones. To define the sequences regulating TIMP expression, we generated transgenic mice that expressed the Escherichia coli beta-galactosidase gene under control of a 5' region of the mouse TIMP gene containing -2158 to -58 bp upstream of the initiator ATG. By use of an in situ assay for beta-galactosidase activity, the TIMP-lacZ fusion gene product was localized to tissues that also expressed the endogenous TIMP gene, such as the mandible, calvaria, and vertebrae. The localization of TIMP to regions of intramembranous and endochondral bone is similar to that previously reported for TGF-beta, a growth modulator believed to be involved in regulation of extracellular matrix (ECM) formation. Thus, the expression of TIMP in these regions is consistent with it playing a role in ECM deposition and...Continue Reading

References

Aug 1, 1989·The Journal of Clinical Investigation·D D DeanJ F Woessner
Oct 1, 1988·Genomics·L J MullinsV M Chapman
Sep 1, 1988·Journal of Virology·A M FlennikenB R Williams
Mar 1, 1985·Collagen and Related Research·H G WelgusG P Stricklin
Dec 1, 1987·The Journal of Cell Biology·U HeineM B Sporn
Dec 1, 1984·Collagen and Related Research·E D HarrisS M Krane
Apr 1, 1981·The Biochemical Journal·T E CawstonJ J Reynolds
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 1, 1958·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M McGEE-RUSSELL

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Citations

Jun 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·I M ClaussL H Glimcher
Sep 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R Cañete-SolerR J Muschel
Jul 1, 1993·Molecular Reproduction and Development·P WaterhouseR Khokha
May 1, 1994·Molecular Reproduction and Development·J K Griffin, S R Blecher
Jan 1, 1993·Journal of Neuro-oncology·U P ThorgeirssonD E Gomez
Sep 1, 1996·In Vitro Cellular & Developmental Biology. Animal·H YegerB R Williams
Apr 23, 2004·Trends in Cell Biology·Nathalie OrtegaZena Werb
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y H LiuR E Maxson
Feb 24, 2001·Plastic and Reconstructive Surgery·S M WarrenM T Longaker
Apr 6, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Z WerbL M Coussens
Jan 26, 1999·Annals of the New York Academy of Sciences·Z Werb, J R Chin
Jul 23, 1999·Annals of the New York Academy of Sciences·L BlavierY A Declerck
Jun 26, 2001·The Journal of Surgical Research·S M WarrenM T Longaker
May 9, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K AirolaU K Saarialho-Kere
Aug 27, 2014·Archives of Biochemistry and Biophysics·Katiucia Batista Silva Paiva, José Mauro Granjeiro
Nov 11, 2018·Clinical Genetics·Julián A Ramírez-CheyneCésar Payán-Gómez
Oct 27, 1999·Canadian Journal of Physiology and Pharmacology·M A ForgetR Béliveau
Jan 31, 1997·The Journal of Biological Chemistry·Y LiaoA Husain
Oct 11, 1996·The Journal of Biological Chemistry·K HammaniY A DeClerck
Jan 20, 1995·The Journal of Biological Chemistry·U I SiresH G Welgus
Oct 28, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lang PanXiaoqun Qin
Sep 7, 2017·Scientific Reports·Bijan AkbariAbdulshakour Mohammadnia
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H Birkedal-HansenJ A Engler
Jun 8, 1996·Annals of the New York Academy of Sciences·Y H LiuR Maxson
Jul 30, 1999·Biochemical and Biophysical Research Communications·T MasakiS Noguchi

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